1684
1684 .
Alicyclobacillus cellulosilyticus Medium
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Paenalicyclobacillus cellulosilyticus DSM 107339
* Modification: plus 20g agar for solid medium
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 55 °C
Main sol. 1684 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Carboxymethylcellulose
, sodium salt
(CMC) |
2.50 | g | 2.5 | - | |
| (NH4)2SO4 | 1.30 | g | 1.3 | 9.838 | |
| KH2PO4 | 0.37 | g | 0.37 | 2.719 | |
| Glucose | 1.00 | g | 1 | 5.551 | |
| Yeast extract | 0.25 | g | 0.25 | - | |
| Tryptone | 0.90 | g | 0.9 | 12.662 | |
|
MgSO4 x 7 H2O
(25%) |
1.00 | ml | 0.25 | 1.014 | |
|
CaCl2 x 2 H2O
(7%) |
1.00 | ml | 0.07 | 0.476 | |
|
FeCl3 x 6 H2O
(2%) |
1.00 | ml | 0.02 | 0.074 | |
| Agar* | 20.00 | g | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust to pH 4.8-5.0 (liquid medium) and pH 5.5 - 6.0 agar medium. Autoclave MgSO4 x 7 H2O solution, CaCl2 x 2 H2O solution and FeCl3 x 6 H2O solution separately. | |||||
| 2 Aseptically add all solutions to the basal medium. | |||||
Metadata
| Last modified: | 27.04.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Molecular composition
* modified
beta
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 20 | 59.465 |
| Carboxymethylcellulose | 2.5 | - |
| (NH4)2SO4 | 1.3 | 9.838 |
| Glucose | 1 | 5.551 |
| Tryptone | 0.9 | 12.662 |
| Show more | ||
