1669
1669 .
SCHAEDLER BROTH (Roth; 5772)
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1669 #
ml
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Casein peptone | 5.66 | g | 5.66 | - | |
| Soy peptone | 1.00 | g | 1 | - | |
| Yeast extract | 5.00 | g | 5 | - | |
| Peptone mixture | 5.00 | g | 5 | - | |
| Glucose | 5.83 | g | 5.83 | 32.36 | |
| K2HPO4 | 0.83 | g | 0.83 | 4.765 | |
| NaCl | 1.66 | g | 1.66 | 28.405 | |
|
Tris
(hydroxymethyl aminomethane) |
3.00 | g | 3 | 24.765 | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| Hemin | 0.01 | g | 0.01 | 0.015 | |
| L-Cysteine HCl x H2O | 0.40 | g | 0.4 | 2.277 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Boil medium under CO2 until the resazurin indicator changes from pink to colourless. Cool down under CO2 and adjust pH to 7.6. Switch to N2 to prevent further lowering of the pH. Fill up into Hungate tubes under N2 and autoclave. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Taxonomic range: | Phages |
| Medium type: | Complex medium |
| Final pH: | 7.6 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Glucose | 5.83 | 32.36 |
| Casein peptone | 5.66 | - |
| Yeast extract | 5 | - |
| Peptone mixture | 5 | - |
| Tris | 3 | 24.765 |
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Cultivation metadata from 16 strains
| pH | 4 - 6 | 1 strain |
| pH | 6 - 8 | 1 strain |
| phyla | 4 | 14 strains |
| oxygen | anaerobe | 11 strains |
| oxygen | facultative anaerobe | 1 strain |
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