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DSMZ

DSMZ 1665 . DESULFAMPLUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1665 #

ml
Equipment needed: Autoclave Hungate tubes Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.0 g 19.704 337.174
MgCl2 x 6 H2O 6.0 g 5.911 29.077
Na2SO4 3.0 g 2.956 20.809
CaCl2 x 2 H2O 0.2 g 0.197 1.34
KCl 0.5 g 0.493 6.608
NH4Cl 0.3 g 0.296 5.526
Wolfe's mineral elixir 2.0 ml - -
HEPES
(SIGMA) 		2.4 g 2.365 9.923
Na2-fumarate 1.0 g 0.985 6.156
Yeast extract
(OXOID) 		0.2 g 0.197 -
Sodium resazurin
(0.1% w/v) 		0.5 ml 4.9e-4 0.002
K-phosphate buffer
(0.5 M, pH 7.0) 		2.0 ml - 1
NaHCO3 1.0 g 0.985 11.728
FeCl2 x 4 H2O
(0.2% w/v in 0.02 N HCl) 		10.0 ml 0.02 0.099
Wolin's vitamin solution (10x) 1.0 ml - -
L-Cysteine HCl x H2O 0.5 g 0.493 2.805
Distilled water 1000.0 ml - -
1 Dissolve ingredients (except phosphate buffer, bicarbonate, ferrous chloride, vitamins and cysteine), adjust pH to 7.2 with NaOH and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add phosphate buffer, ferrous chloride, vitamins and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere and bicarbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins should be sterilized by filtration. Adjust pH of the complete medium to 7.2 - 7.5 , if necessary.

Wolfe's mineral elixir #

1000
Solution occurs in 22 other media (first defined in Medium 792).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 30.00 g 30 121.716
MnSO4 x H2O 5.00 g 5 29.583
NaCl 10.00 g 10 171.116
FeSO4 x 7 H2O 1.00 g 1 3.597
CoCl2 x 6 H2O 1.80 g 1.8 13.863
CaCl2 x 2 H2O 1.00 g 1 6.802
ZnSO4 x 7 H2O 1.80 g 1.8 6.26
CuSO4 x 5 H2O 0.10 g 0.1 0.401
AlK(SO4)2 x 12 H2O 0.18 g 0.18 0.379
H3BO3 0.10 g 0.1 1.617
Na2MoO4 x 2 H2O 0.10 g 0.1 0.413
(NH4)2Ni(SO4)2 x 6 H2O 2.80 g 2.8 7.089
Na2WO4 x 2 H2O 0.10 g 0.1 0.303
Na2SeO4 0.10 g 0.1 0.529
Distilled water 1000.00 ml - -
1 First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts.

Wolin's vitamin solution (10x) #

1000
Solution occurs in 308 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 12.05.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2 - 7.5
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 5.911 29.077
Na2SO4 2.956 20.809
HEPES 2.365 9.923
NaHCO3 0.985 11.728
Na2-fumarate 0.985 6.156
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain