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DSMZ

DSMZ 165 . ACETIVIBRIO CELLULOLYTICUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 165 #

ml
Equipment needed: Autoclave Filter Gassing station Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
Mineral solution 1 75.00 ml - -
Mineral solution 2 75.00 ml - -
Modified Wolin's mineral solution 10.00 ml - -
FeSO4 x 7 H2O solution (0.1% w/v) 20.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 0.002
Na2CO3 1.50 g 1.499 14.138
Cellobiose 3.00 g 2.997 8.755
Cellulose
(MN300)
optional 		3.00 g 2.997 -
Wolin's vitamin solution (10x) 1.00 ml - -
L-Cysteine HCl x H2O 0.25 g 0.25 1.422
Na2S x 9 H2O 0.25 g 0.25 1.04
Distilled water 820.00 ml - -
1 Dissolve ingredients except carbonate, cellobiose, vitamins, cysteine and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes and autoclave. Add cellobiose, vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Cellobiose and vitamins have to be sterilized by filtration. Prior to use adjust pH of complete medium to 7.2 - 7.4. Cellulose (MN 300, Whatman CF II, Kleenex tissue paper, or HCl treated cotton) can be used as an alternative to cellobiose.

Mineral solution 1 #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 3.9 g 3.9 22.391
Distilled water 1000.0 ml - -

Mineral solution 2 #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 2.40 g 2.4 17.636
(NH4)2SO4 6.00 g 6 45.406
NaCl 0.59 g 0.59 10.096
MgSO4 x 7 H2O 1.20 g 1.2 4.869
CaCl2 x 2 H2O 0.72 g 0.72 4.897
Distilled water 1000.00 ml - -

Modified Wolin's mineral solution #

1000
Solution occurs in 168 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

FeSO4 x 7 H2O solution (0.1% w/v) #

1000
Solution occurs in 13 other media (first defined in Medium 119).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 1 g 1 3.597
H2SO4
(0.1 N) 		1000 ml - -
1 The ferrous sulfate solution is not stable and should be freshly prepared.

Wolin's vitamin solution (10x) #

1000
Solution occurs in 308 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 09.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2 - 7.4
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Cellobiose 2.997 8.755
Cellulose
optional 		2.997 -
Na2CO3 1.499 14.138
(NH4)2SO4 0.45 3.402
K2HPO4 0.292 1.678
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
oxygen obligate anaerobe 1 strain
temperature mesophilic 1 strain