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DSMZ

DSMZ 1633 . V2 MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1633 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone
(Bacto) 		0.250 g 0.25 -
Yeast extract
(Bacto) 		0.250 g 0.25 -
HEPES 2.380 g 2.38 9.987
Mineral salts solution 20.000 ml - -
Trace element solution 1.000 ml - -
NaHCO3 0.025 g 0.025 0.298
CaCO3 0.025 g 0.025 0.25
CaCl2 x 2 H2O 0.015 g 0.015 0.102
MgSO4 x 7 H2O 0.049 g 0.049 0.199
KNO3 0.010 g 0.01 0.099
KH2PO4 1.400 mg 0.001 0.01
NH4Cl 0.530 mg 5.3e-4 0.01
Agar
(Bacto) 		15.000 g 15 -
Double distilled water 956.000 ml - -
1 Sterilize 20 min./121°C. After cooling to 60°C add:
N-Acetylglucosamine
(10% stock solution) 		8.000 ml 0.8 3.616
Glucose
(2.5%, sterile-filtered) 		10.000 ml 0.25 1.388
Vitamin solution (double conc.)
(medium 621) 		5.000 ml - -
2 Adjust pH to 7.0 with 40% KOH.

Mineral salts solution #

1000
Solution occurs in 4 other media (first defined in Medium 1560).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA) 		10.00 g 10 52.318
MgSO4 x 7 H2O 29.70 g 29.7 120.499
CaCl2 x 2 H2O 3.34 g 3.34 22.719
Na2MoO7O4 x 2 H2O 12.67 mg 0.013 -
FeSO4 x 7 H2O 99.00 mg 0.099 0.356
"Metals 44" 50.00 ml - -
Distilled water 950.00 ml - -
1 Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4.

"Metals 44" #

1000
Solution occurs in 4 other media (first defined in Medium 1560).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-EDTA 250.0 mg 0.25 0.744
ZnSO4 x 7 H2O 1095.0 mg 1.095 3.808
FeSO4 x 7 H2O 500.0 mg 0.5 1.798
MnSO4 x H2O 154.0 mg 0.154 0.911
CuSO4 x 5 H2O 39.2 mg 0.039 0.157
Co(NO3)2 x 6 H2O 24.8 mg 0.025 0.085
Na2B4O7 x 10 H2O 17.7 mg 0.018 -
Distilled water 1000.0 ml - -

Trace element solution #

1000
Solution occurs in 4 other media (first defined in Medium 1560).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-Nitrilotriacetat 1.50 g 1.5 -
MnSO4 x H2O 0.50 g 0.5 2.958
Fe SO4 x 7 H2O 0.10 g 0.1 0.36
Co(NO3)2 x 6 H2O 0.10 g 0.1 0.344
ZnCl2 0.10 g 0.1 0.734
NiCl2 x 6 H2O 0.05 g 0.05 0.386
H2SeO3 0.05 g 0.05 0.388
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.01 g 0.01 0.021
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Na2WO4 x 2 H2O 0.01 g 0.01 0.03
Double distilled water 1000.00 ml - -

Vitamin solution (double conc.) #

1000
Solution occurs in 4 other media (first defined in Medium 1560).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 4.0 mg 0.004 0.016
Folic acid 4.0 mg 0.004 0.009
Pyridoxine hydrochloride 20.0 mg 0.02 0.097
Riboflavine 10.0 mg 0.01 0.027
Thiamine-HCl x 2 H2O 10.0 mg 0.01 0.027
Nicotinamide 10.0 mg 0.01 0.082
D-Calcium pantothenate 10.0 mg 0.01 -
Vitamin B12 0.2 mg 2.0e-4 1.5e-4
p-Aminobenzoic acid 10.0 mg 0.01 0.073
Double distilled water 1000.0 ml - -

Metadata

Last modified: 07.11.25
Source: DSMZ
Medium type: Complex medium
Final pH: 7.0
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 -
HEPES 2.38 9.987
N-Acetylglucosamine 0.8 3.616
Glucose 0.25 1.388
Peptone 0.25 -
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Cultivation metadata from 2 strains BacDive

phyla 1 1 strain