DSMZ 162 . HYPHOMICROBIUM MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Hyphomicrobium sp. DSM 6363

This strain uses the standard medium without modifications.


Growth conditions: aerobic 30 °C
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.36 g 1.36 9.994
Na2HPO4 2.13 g 2.13 15.004
(NH4)2SO4 0.50 g 0.5 3.784
MgSO4 x 7 H2O 0.20 g 0.2 0.811
CaCl2 x 2 H2O 6.00 mg 0.006 0.041
FeSO4 x 7 H2O 3.00 mg 0.003 0.011
MnSO4 x H2O 1.00 mg 1.0e-3 0.006
Na2MoO4 x 2 H2O 1.50 mg 0.002 0.006
Methylamine hydrochloride 3.38 g 3.38 50.059
Yeast extract 0.10 g 0.1 -
Distilled water 1000.00 ml - -
1 Adjust pH to 7.4 with NaOH. Autoclave 20 min at 121°C; final pH: 7.2.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 7
Strains with modifications: 1
Hyphomicrobium sp. DSM 6363

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Methylamine hydrochloride 3.38 50.059
Na2HPO4 2.13 15.004
KH2PO4 1.36 9.994
(NH4)2SO4 0.5 3.784
MgSO4 x 7 H2O 0.2 0.811
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Cultivation metadata from 7 strains BacDive

phyla 1 7 strains
temperature mesophilic 7 strains