1629
1629 .
MINIMAL MEDIA CONTAINING METHANOL
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
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Medium for Methylobacterium sp. DSM 102640
This strain uses the standard medium without modifications.
Growth conditions: 2-3 days
aerobic
28 °C
Main sol. 1629 #
ml
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 1.62 | g | 1.62 | 30.286 | |
| MgSO4 x 7 H2O | 0.20 | g | 0.2 | 0.811 | |
| K2HPO4 | 2.40 | g | 2.4 | 13.779 | |
| NaH2PO4 x 2 H2O | 1.10 | g | 1.1 | 9.168 | |
| Na2-EDTA | 15.00 | mg | 0.015 | 0.045 | |
| FeSO4 x 6 H2O | 3.00 | mg | 0.003 | 0.02 | |
| ZnSO4 x 7 H2O | 4.50 | mg | 0.005 | 0.016 | |
| CoCl2 x 6 H2O | 3.00 | mg | 0.003 | 0.023 | |
| MnCl2 x 4 H2O | 0.64 | mg | 6.4e-4 | 0.003 | |
| H3BO3 | 1.00 | mg | 1.0e-3 | 0.016 | |
| Na2MoO4 x 2 H2O | 0.40 | mg | 4.0e-4 | 0.002 | |
| CuSO4 x 2 H2O | 0.30 | mg | 3.0e-4 | 0.002 | |
| CaCl2 x 6 H2O | 3.00 | mg | 0.003 | 0.014 | |
| Methanol | 5.00 | ml | 3.96 | 123.588 | |
| Agar | 15.00 | g | 15 | 44.599 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Prepare the medium without methanol. Adjust pH to 7.1. | |||||
| 2 Autoclave and after cooling to 50°C add the methanol from a filter-sterilized stock solution. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 7.1 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 12 |
Methylobacterium sp. DSM 102640
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Methanol | 3.96 | 123.588 |
| K2HPO4 | 2.4 | 13.779 |
| NH4Cl | 1.62 | 30.286 |
| NaH2PO4 x 2 H2O | 1.1 | 9.168 |
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Cultivation metadata from 12 strains
| phyla | 1 | 12 strains |
| oxygen | obligate aerobe | 5 strains |
| oxygen | aerobe | 1 strain |