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DSMZ

DSMZ 1566 . HAHA100 V MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1566 #

ml
Solution occurs in 1 other media (first defined in Medium 1566).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 26.37 g - -
MgSO4 x 7 H2O 6.80 g - -
MgCl2 x 6 H2O 5.67 g - -
CaCl2 x 2 H2O 1.47 g - -
KCl 0.72 g - -
KBr 0.10 g - -
NH4Cl 0.80 mg - -
KH2PO4 0.02 g - -
H3BO3 0.02 g - -
SrCl2 0.02 g - -
NaF 3.00 mg - -
Tryptone peptone 0.10 g - -
Casamino acids
(Oxoid) 		0.10 g - -
Yeast extract 0.10 g - -
Micronutrient solution SL 8
(medium 1162) 		2.00 ml - -
Distilled water 982.00 ml - -
1 Autoclave at 121 °C for 20 min.
2 Before inoculation add from sterile stock solutions:
α-D-Glucose monohydrate
(10% in H2O) 		1.00 ml - -
Cellobiose
(10% in H2O) 		1.00 ml - -
Standard vitamin solution 10.00 ml - -
NaHCO3
(1M in H2O) 		2.00 ml - -
Selenite-tungstate solution 2.00 ml - -
3 Adjust pH to 7.5 with 1 M HCl or 1 M NaHCO3

Selenite-tungstate solution #

1000
Solution occurs in 180 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Standard vitamin solution #

100
Solution occurs in 3 other media (first defined in Medium 428).
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Riboflavin 10.0 mg 0.1 0.266
Thiamine-HCl x 2 H2O 50.0 mg 0.5 1.339
Nicotinic acid 50.0 mg 0.5 4.061
Pyridoxine hydrochloride 50.0 mg 0.5 2.431
Calcium pantothenate 50.0 mg 0.5 1.049
Biotin 0.1 mg 1.0e-3 0.004
Folic acid 0.2 mg 0.002 0.005
Vitamin B12 1.0 mg 0.01 0.007
Distilled water 100.0 ml - -
1 Filter sterilize (0.2 µm pore-size) and store at 4°C.

Micronutrient solution SL 8 #

1000
Solution occurs in 2 other media (first defined in Medium 1162).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 5.2 g 5.2 15.467
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 62.0 mg 0.062 1.003
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 17.0 mg 0.017 0.1
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 1000.0 ml - -

Metadata

Last modified: 03.03.22
Source: DSMZ
Synonyms: HAHA100 mod V2- MEDIUM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 5
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 26.37 451.232
MgSO4 x 7 H2O 6.8 27.589
MgCl2 x 6 H2O 5.67 27.889
CaCl2 x 2 H2O 1.47 9.999
KCl 0.72 9.658
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Cultivation metadata from 5 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 3 5 strains
oxygen aerobe 1 strain
oxygen obligate aerobe 1 strain
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