1531 .
PLANCTOMYCES PYREFORMIS MEDIUM
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Equipment needed:
Final pH:
5.8 - 6.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
N-Acetylglucosamine | 1.00 | g | ||
|
|
KH2PO4 | 0.10 | g | ||
|
|
(NH4)2SO4 | 0.20 | g | ||
|
|
MgSO4 x 7 H2O | 0.10 | g | ||
|
|
CaCl2 x 2 H2O | 0.02 | g | ||
|
|
Hutners basal salts | 1.00 | ml | ||
|
|
Vitamin solution (double conc.) | 0.10 | g | ||
|
|
Yeast extract | 0.20 | g | ||
|
|
Glucose | 0.50 | g | ||
|
|
Agar
(Difco) |
18.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 5.8 - 6.5. | |||||
Hutners basal salts #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid
(NTA) |
10.00 | g | ||
|
|
MgSO4 x 7 H2O | 29.70 | g | ||
|
|
CaCl2 x 2 H2O | 3.34 | g | ||
|
|
(NH4)6Mo7O24 x 4 H2O | 9.25 | mg | ||
|
|
FeSO4 x 7 H2O | 99.00 | mg | ||
|
|
"Metals 44" | 50.00 | ml | ||
|
|
Distilled water | 950.00 | ml | ||
| 1 Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4. | |||||
"Metals 44" #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na-EDTA | 250.0 | mg | ||
|
|
ZnSO4 x 7 H2O | 1095.0 | mg | ||
|
|
FeSO4 x 7 H2O | 500.0 | mg | ||
|
|
MnSO4 x H2O | 154.0 | mg | ||
|
|
CuSO4 x 5 H2O | 39.2 | mg | ||
|
|
Co(NO3)2 x 6 H2O | 24.8 | mg | ||
|
|
Na2B4O7 x 10 H2O | 17.7 | mg | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions. | |||||
Vitamin solution (double conc.) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Biotin | 4.0 | mg | ||
|
|
Folic acid | 4.0 | mg | ||
|
|
Pyridoxine hydrochloride | 20.0 | mg | ||
|
|
Riboflavine | 10.0 | mg | ||
|
|
Thiamine-HCl x 2 H2O | 10.0 | mg | ||
|
|
Nicotinamide | 10.0 | mg | ||
|
|
D-Calcium pantothenate | 10.0 | mg | ||
|
|
Vitamin B12 | 0.2 | mg | ||
|
|
p-Aminobenzoic acid | 10.0 | mg | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Store in the dark and cold (5°C). | |||||