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DSMZ

DSMZ 1531 . PLANCTOMYCES PYREFORMIS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Paludibaculum fermentans DSM 26340

This strain uses the standard medium without modifications.

Growth conditions: 25 °C

Main sol. 1531 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
N-Acetylglucosamine 1.00 g 1 4.521
KH2PO4 0.10 g 0.1 0.735
(NH4)2SO4 0.20 g 0.2 1.514
MgSO4 x 7 H2O 0.10 g 0.1 0.406
CaCl2 x 2 H2O 0.02 g 0.02 0.136
Hutners basal salts 1.00 ml - -
Vitamin solution (double conc.) 0.10 g 0.1 -
Yeast extract 0.20 g 0.2 -
Glucose 0.50 g 0.5 2.775
Agar
(Difco) 		18.00 g 18 53.519
Distilled water 1000.00 ml - -
1 Adjust pH to 5.8 - 6.5.

Hutners basal salts #

1000
Solution occurs in 3 other media (first defined in Medium 1144).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA) 		10.00 g 10 52.318
MgSO4 x 7 H2O 29.70 g 29.7 120.499
CaCl2 x 2 H2O 3.34 g 3.34 22.719
(NH4)6Mo7O24 x 4 H2O 9.25 mg 0.009 -
FeSO4 x 7 H2O 99.00 mg 0.099 0.356
"Metals 44" 50.00 ml - -
Distilled water 950.00 ml - -
1 Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4.

"Metals 44" #

1000
Solution occurs in 34 other media (first defined in Medium 590).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-EDTA 250.0 mg 0.25 0.744
ZnSO4 x 7 H2O 1095.0 mg 1.095 3.808
FeSO4 x 7 H2O 500.0 mg 0.5 1.798
MnSO4 x H2O 154.0 mg 0.154 0.911
CuSO4 x 5 H2O 39.2 mg 0.039 0.157
Co(NO3)2 x 6 H2O 24.8 mg 0.025 0.085
Na2B4O7 x 10 H2O 17.7 mg 0.018 -
Distilled water 1000.0 ml - -
1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.

Vitamin solution (double conc.) #

1000
Solution occurs in 6 other media (first defined in Medium 621).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 4.0 mg 0.004 0.016
Folic acid 4.0 mg 0.004 0.009
Pyridoxine hydrochloride 20.0 mg 0.02 0.097
Riboflavine 10.0 mg 0.01 0.027
Thiamine-HCl x 2 H2O 10.0 mg 0.01 0.027
Nicotinamide 10.0 mg 0.01 0.082
D-Calcium pantothenate 10.0 mg 0.01 -
Vitamin B12 0.2 mg 2.0e-4 1.5e-4
p-Aminobenzoic acid 10.0 mg 0.01 0.073
Distilled water 1000.0 ml - -
1 Store in the dark and cold (5°C).

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 5.8 - 6.5
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Associated strains

All strains for this medium: 2
Paludibaculum fermentans DSM 26340

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 18 53.519
N-Acetylglucosamine 1 4.521
Glucose 0.5 2.775
(NH4)2SO4 0.2 1.514
Yeast extract 0.2 -
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Cultivation metadata from 2 strains BacDive

phyla 2 2 strains
oxygen microaerophile 1 strain
temperature mesophilic 2 strains