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DSMZ

DSMZ 1513 . SULFURIMONAS GOTLANDICA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1513 #

ml
Equipment needed: Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 5.55 g 5.539 94.78
MgCl2 x 6 H2O 2.29 g 2.285 11.242
CaCl2 x 2 H2O 0.34 g 0.339 2.308
KCl 0.15 g 0.15 2.008
Na2SO4 0.91 g 0.908 6.394
KNO3 1.01 g 1.008 9.97
HEPES buffer
(SIGMA) 		2.38 g 2.375 9.967
NH4Cl 0.05 g 0.05 0.933
SrCl2
(0.1% w/v) 		8.00 ml 0.008 0.05
H3BO3
(0.1% w/v) 		6.00 ml 0.006 0.097
NaF
(0.1% w/v) 		4.00 ml 0.004 0.095
K2HPO4
(0.1% w/v) 		1.00 ml 1.0e-3 0.006
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
NaHCO3 0.20 g 0.2 2.376
Na2S2O3 x 5 H2O 2.48 g 2.475 9.973
Wolin's vitamin solution (10x) 1.00 ml - -
Distilled water 980.00 ml - -
1 Dissolve ingredients (except bicarbonate, thiosulfate and vitamins) and adjust pH to 7.3. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic serum vials (e.g., Balch-type tubes) and autoclave. Add thiosulfate and vitamins from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Stock solutions of vitamins and thiosulftae should be sterilized by filtration. Adjust pH of the complete medium to 7.3 - 7.5, if necessary.
2 After inoculation pressurize vials to 0.7 bar overpressure with sterile 100% N2 gas.

Trace element solution SL-10 #

1000
Solution occurs in 265 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Selenite-tungstate solution #

1000
Solution occurs in 178 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Wolin's vitamin solution (10x) #

1000
Solution occurs in 305 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 20.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.3 - 7.5
Equipment needed: Autoclave, Filter, Gassing station
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 5.539 94.78
Na2S2O3 x 5 H2O 2.475 9.973
HEPES buffer 2.375 9.967
MgCl2 x 6 H2O 2.285 11.242
KNO3 1.008 9.97
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
oxygen facultative anaerobe 1 strain
temperature psychrophilic 1 strain