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DSMZ

DSMZ 1508 . CALDERIHABITANS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1508 #

ml
Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Sea Salt
(SIGMA, must not show signs of caking) 		21.00 g 20.958 -
K2HPO4 0.10 g 0.1 0.573
NH4Cl 0.10 g 0.1 1.866
NaNO3 0.05 g 0.05 0.587
Yeast extract 0.05 g 0.05 -
Ferric citrate monohydrate 0.01 g 0.01 0.038
Trace element solution SL-4 1.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 0.002
Na2CO3 1.50 g 1.497 14.124
Na-pyruvate 1.00 g 0.998 9.069
Na2S2O3 x 5 H2O 1.00 g 0.998 4.021
Wolin's vitamin solution (10x) 1.00 ml - -
Na2S x 9 H2O 0.25 g 0.25 1.039
Distilled water 1000.00 ml - -
1 Dissolve ingredients except carbonate, pyruvate, thiosulfate, vitamins and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add pyruvate, thiosulfate, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Stock solutions of pyruvate, thiosulfate and vitamins should be sterilized by filtration. Prior to use adjust pH of complete medium to 7.3 - 7.5, if necessary.

Trace element solution SL-4 #

1000
Solution occurs in 23 other media (first defined in Medium 14).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 0.50 g 0.5 1.487
FeSO4 x 7 H2O 0.20 g 0.2 0.719
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.

Wolin's vitamin solution (10x) #

1000
Solution occurs in 308 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 29.04.25
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.3 - 7.5
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sea Salt 20.958 -
Na2CO3 1.497 14.124
Na-pyruvate 0.998 9.069
Na2S2O3 x 5 H2O 0.998 4.021
Na2S x 9 H2O 0.25 1.039
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
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