1492 .
HALF STRENGTH R2A
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Equipment needed:
Autoclave
Final pH:
7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast extract | 0.250 | g | ||
|
|
Proteose peptone
(Difco no. 3) |
0.250 | g | ||
|
|
Casamino acids | 0.250 | g | ||
|
|
Glucose | 0.250 | g | ||
|
|
Starch
(soluble) |
0.250 | g | ||
|
|
Na-pyruvate | 0.250 | g | ||
|
|
K2HPO4 | 0.150 | g | ||
|
|
MgSO4 x 7 H2O | 0.025 | g | ||
|
|
Distilled water | 1000.000 | ml | ||
| 1 Adjust pH to 7.2 with K2HPO4 or KH2PO4. | |||||
| 2 Add agar after adjusting the pH, then boil to dissolve the agar | |||||
|
|
Agar | 15.000 | g | ||
| 3 Autoclave at 121 °C for 15 min. | |||||