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DSMZ

DSMZ 1479 . HALODESULFURIARCHAEUM MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1479 #

ml
Equipment needed: Hungate tubes Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 240.0 g 240 4106.78
K2HPO4 2.5 g 2.5 14.353
(NH4)2SO4 0.5 g 0.5 3.784
HEPES 10.0 g 10 41.964
Trace element solution 1.0 ml - -
Sulfur powder 2.0 g 2 7.797
Distilled water 1000.0 ml - -
1 Prepare the liquid medium. Dispense the medium into Hungate tubes or serum bottles under gassing with N2. Steam medium for 3h on each of 3 successive days. Before inoculation add from sterile stock solutions:
MgCl2 x 6 H2O 3.0 mM 0.61 3
NaHCO3 5.0 mM 0.42 5
Sodium formate 50.0 mM 3.4 50
Yeast extract 0.2 g/l 0.2 -
Vitamin mix (pfennig & Lippert,1966) 1.0 ml - -
Disodium sulfide
(1M filter sterilized stock) 		1.0 mM - 1

Trace element solution #

1000
Solution occurs in 3 other media (first defined in Medium 1478).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.00 g 2 7.194
ZnSO4 0.10 g 0.1 0.62
MnCl2 0.03 g 0.03 0.238
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 5 H2O 0.01 g 0.01 0.045
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.02 g 0.02 0.083
Distilled water 1000.00 ml - -
1 Adjust pH to 3.0-4.0 with HCl.
2 Autoclave at 121 °C for 15 min.
3 Note: autoclave in closed bottles

Vitamin mix (pfennig & Lippert,1966) #

400
Solution occurs in 1 other media (first defined in Medium 1479).
Equipment needed: Filter
1 1: acidic solution (HCl, pH3; 100ml)
Compound Amount Unit Conc. [g/L] Conc. [mM]
Thiamin 5.0 mg 0.013 0.047
Calcium pantothenate 5.0 mg 0.013 0.026
2 2: basic solution (100ml 0.1N NaOH)
Biotin 2.0 mg 0.005 0.02
PABA
(p-aminobenzoic acid) 		5.0 mg 0.013 0.091
Nicotinic acid 5.0 mg 0.013 0.102
Pyridoxine 10.0 mg 0.025 0.148
3 3: Neutral solution in 100 ml demi water
Folic acid 2.0 mg 0.005 0.011
Riboflavin 5.0 mg 0.013 0.033
4 4: neutral solution in 100 ml demi water
B12 0.5 mg 0.001 9.2e-4
5 Sterilize by filtration.
6 Mix 1:1:1:1 just after addition or add separatlely, each 1ml/L.

Metadata

Last modified: 24.02.22
Source: DSMZ
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave, Filter, Hungate tubes
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 240 4106.78
HEPES 10 41.964
Sodium formate 3.4 50
K2HPO4 2.5 14.353
Sulfur powder 2 7.797
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