MediaDive DSMZ Digital Diversity
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder SPARQL endpoint Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 1479 . HALODESULFURIARCHAEUM MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1479 #

ml
Equipment needed: Hungate tubes Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 240.0 g 240 4106.78
K2HPO4 2.5 g 2.5 14.353
(NH4)2SO4 0.5 g 0.5 3.784
HEPES 10.0 g 10 41.964
Trace element solution 1.0 ml - -
Sulfur powder 2.0 g 2 7.797
Distilled water 1000.0 ml - -
1 Prepare the liquid medium. Dispense the medium into Hungate tubes or serum bottles under gassing with N2. Steam medium for 3h on each of 3 successive days. Before inoculation add from sterile stock solutions:
MgCl2 x 6 H2O 3.0 mM 0.61 3
NaHCO3 5.0 mM 0.42 5
Sodium formate 50.0 mM 3.4 50
Yeast extract 0.2 g/l 0.2 -
Vitamin mix (pfennig & Lippert,1966) 1.0 ml - -
Disodium sulfide
(1M filter sterilized stock) 		1.0 mM - 1

Trace element solution #

1000
Solution occurs in 5 other media (first defined in Medium 1478).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.00 g 2 7.194
ZnSO4 0.10 g 0.1 0.62
MnCl2 0.03 g 0.03 0.238
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 5 H2O 0.01 g 0.01 0.045
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.02 g 0.02 0.083
Distilled water 1000.00 ml - -
1 Adjust pH to 3.0-4.0 with HCl.
2 Autoclave at 121 °C for 15 min.
3 Note: autoclave in closed bottles

Vitamin mix (pfennig & Lippert,1966) #

400
Solution occurs in 1 other media (first defined in Medium 1479).
Equipment needed: Filter
1 1: acidic solution (HCl, pH3; 100ml)
Compound Amount Unit Conc. [g/L] Conc. [mM]
Thiamin 5.0 mg 0.013 0.047
Calcium pantothenate 5.0 mg 0.013 0.026
2 2: basic solution (100ml 0.1N NaOH)
Biotin 2.0 mg 0.005 0.02
PABA
(p-aminobenzoic acid) 		5.0 mg 0.013 0.091
Nicotinic acid 5.0 mg 0.013 0.102
Pyridoxine 10.0 mg 0.025 0.148
3 3: Neutral solution in 100 ml demi water
Folic acid 2.0 mg 0.005 0.011
Riboflavin 5.0 mg 0.013 0.033
4 4: neutral solution in 100 ml demi water
B12 0.5 mg 0.001 9.2e-4
5 Sterilize by filtration.
6 Mix 1:1:1:1 just after addition or add separatlely, each 1ml/L.

Metadata

Last modified: 24.02.22
Source: DSMZ
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave, Filter, Hungate tubes
Edit in Medium builder

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 240 4106.78
HEPES 10 41.964
Sodium formate 3.4 50
K2HPO4 2.5 14.353
Sulfur powder 2 7.797
Show more
CSV
JSON