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DSMZ

DSMZ 146 . HALOCOCCUS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 146 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 500 ml - -
Solution B 100 ml - -
Solution C 400 ml - -
1 Steam the three solutions separately, mix together solutions B and C and adjust pH to 8.4. Autoclave the skim milk at 112°C for 15 min, and the agar salt mixture at 121°C for 20 min. Add the hot mixture to the warm skim milk aseptically.

Solution A #

500
Compound Amount Unit Conc. [g/L] Conc. [mM]
Skim milk
(Difco) 		50 g 100 -
Distilled water 500 ml - -

Solution B #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 10 g 100 405.719
KNO3 2 g 20 197.818
NaCl 200 g 2000 34223.1
Fe(III) citrate trace - -
Distilled water 100 ml - -
1 Use trace of Fe(III)citrate.

Solution C #

400
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Neopeptone 5 g 12.5 -
Glycerol 10 g 25 271.474
Agar 25 g 62.5 185.829
Distilled water 400 ml - -

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 8.4
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 200 3422.31
Skim milk 50 -
Agar 25 74.332
Glycerol 10 108.59
MgSO4 x 7 H2O 10 40.572
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
temperature mesophilic 3 strains