Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
K2HPO4 | 0.23 | g | 0.229 | 1.313 | |
KH2PO4 | 0.23 | g | 0.229 | 1.68 | |
NaCl | 0.45 | g | 0.447 | 7.654 | |
(NH4)2SO4 | 0.45 | g | 0.447 | 3.385 | |
CaCl2 x 2 H2O | 0.06 | g | 0.06 | 0.406 | |
MgSO4 x 7 H2O | 0.09 | g | 0.089 | 0.363 | |
Brain heart infusion
(BD BBL) |
6.00 | g | 5.964 | - | |
Yeast extract | 5.00 | g | 4.97 | - | |
Volatile fatty acid mixture | 3.10 | ml | - | - | |
Haemin solution
(0.05% w/v) |
2.00 | ml | - | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
NaHCO3 | 6.40 | g | 6.362 | 75.73 | |
Disodium succinate | 5.00 | g | 4.97 | 30.671 | |
Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
L-Cysteine HCl x H2O | 0.30 | g | 0.298 | 1.698 | |
Na2S x 9 H2O | 0.30 | g | 0.298 | 1.242 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients except bicarbonate, succinate, vitamins, cysteine and sulfide, then sparge medium with 100% CO2 gas for 30 - 45 min to make it anoxic. Add the bicarbonate and equilibrate the medium with the CO2 gas to reach a pH between 6.5 and 7.0. Dispense medium under 100% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add succinate, vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The vitamin solution should be sterilized by filtration. Prior to use adjust pH of complete medium to 6.6 - 6.8, if necessary. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Acetic acid | 548.50 | ml | 573.182 | 9545.09 |
Propionic acid | 193.50 | ml | 192.145 | 2593.76 |
Butyric acid | 129.00 | ml | - | - |
n-Valeric acid | 32.25 | ml | 30.283 | 296.512 |
iso-Butyric acid | 32.25 | ml | 30.638 | 347.719 |
DL-2-Methylbutyric acid | 32.25 | ml | - | - |
iso-Valeric acid | 32.25 | ml | 30.025 | 293.986 |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Haemin | 50 | mg | 0.5 | 0.767 | |
NaOH
(1 N) |
1 | ml | - | - | |
Distilled water | 100 | ml | - | - | |
1 Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 20 | mg | 0.02 | 0.082 |
Folic acid | 20 | mg | 0.02 | 0.045 |
Pyridoxine hydrochloride | 100 | mg | 0.1 | 0.486 |
Thiamine HCl | 50 | mg | 0.05 | 0.148 |
Riboflavin | 50 | mg | 0.05 | 0.133 |
Nicotinic acid | 50 | mg | 0.05 | 0.406 |
Calcium D-(+)-pantothenate | 50 | mg | 0.05 | 0.21 |
Vitamin B12 | 1 | mg | 1.0e-3 | 7.4e-4 |
p-Aminobenzoic acid | 50 | mg | 0.05 | 0.365 |
(DL)-alpha-Lipoic acid | 50 | mg | 0.05 | 0.242 |
Distilled water | 1000 | ml | - | - |
Last modified: | 08.09.22 |
Source: | DSMZ |
Medium type: | Complex medium |
Final pH: | 6.6 - 6.8 |
Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
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Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .