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DSMZ

DSMZ 1444 . PIRELLULA MEDIUM (M30a)

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1444 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution 1 950 ml - -
Solution 2 50 ml - -

Solution 1 #

950
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Hutner's salts 20 ml - -
Tris-HCl buffer
(0.1M, pH 7.5) 		50 ml - 5
Artifical sea water 500 ml - -
Agar 18 g 18.947 56.336
Distilled water 380 ml - -
1 Adjust pH to 7.5, autoclave at 121°C for 20 min.
2 For agar plates increase the pH of the Tris/HCl buffer to 8.5.

Solution 2 #

50
Solution occurs in 1 other media (first defined in Medium 1443).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
N-Acetylglucosamine 2.0 g 40 180.824
Na2HPO4 x 2 H2O 0.1 g 2 14.089
Vitamin solution (double conc.) 10.0 ml - -
Distilled water 40.0 ml - -
1 Adjust pH to 7.5, filter-sterilize, and add to solution 1.

Hutner's salts #

1000
Solution occurs in 24 other media (first defined in Medium 590).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA) 		10.000 g 10 52.318
MgSO4 x 7 H2O 29.700 g 29.7 120.499
CaCl2 x 2 H2O 3.335 g 3.335 22.685
(NH4)6Mo7O24 x 4 H2O 9.250 mg 0.009 0.007
FeSO4 x 7 H2O 99.000 mg 0.099 0.356
"Metals 44" 50.000 ml - -
Distilled water 950.000 ml - -
1 Dissolve the nitrilotriacetic acid.
2 Adjust pH to 7.0 with KOH (~7.3 g).
3 Dissolve other salts separately and combine.
4 Adjust pH to 6.8 with NaOH or H2SO4.

Artifical sea water #

1000
Solution occurs in 2 other media (first defined in Medium 600).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 23.47 g 23.47 401.608
Na2SO4 3.92 g 3.92 27.598
MgCl2 x 6 H2O 10.64 g 10.64 52.336
CaCl2 1.10 g 1.1 9.912
NaHCO3 192.00 mg 0.192 2.286
KCl 664.00 mg 0.664 8.907
KBr 96.00 mg 0.096 0.807
H3BO3 26.00 mg 0.026 0.42
SrCl2 24.00 mg 0.024 0.151
NaF 3.00 mg 0.003 0.071
Distilled water 1000.00 ml - -

Vitamin solution (double conc.) #

1000
Solution occurs in 6 other media (first defined in Medium 621).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 4.0 mg 0.004 0.016
Folic acid 4.0 mg 0.004 0.009
Pyridoxine hydrochloride 20.0 mg 0.02 0.097
Riboflavine 10.0 mg 0.01 0.027
Thiamine-HCl x 2 H2O 10.0 mg 0.01 0.027
Nicotinamide 10.0 mg 0.01 0.082
D-Calcium pantothenate 10.0 mg 0.01 -
Vitamin B12 0.2 mg 2.0e-4 1.5e-4
p-Aminobenzoic acid 10.0 mg 0.01 0.073
Distilled water 1000.0 ml - -
1 Store in the dark and cold (5°C).

"Metals 44" #

1000
Solution occurs in 34 other media (first defined in Medium 590).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-EDTA 250.0 mg 0.25 0.744
ZnSO4 x 7 H2O 1095.0 mg 1.095 3.808
FeSO4 x 7 H2O 500.0 mg 0.5 1.798
MnSO4 x H2O 154.0 mg 0.154 0.911
CuSO4 x 5 H2O 39.2 mg 0.039 0.157
Co(NO3)2 x 6 H2O 24.8 mg 0.025 0.085
Na2B4O7 x 10 H2O 17.7 mg 0.018 -
Distilled water 1000.0 ml - -
1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.

Metadata

Last modified: 22.02.22
Source: DSMZ
Medium type: Defined medium
Final pH: 7.5
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 18 53.519
NaCl 11.735 200.804
MgCl2 x 6 H2O 5.32 26.168
N-Acetylglucosamine 2 9.041
Na2SO4 1.96 13.799
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