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DSMZ

DSMZ 1441 . ALKALIPHILES PIRELLULA MEDIUM (M1)

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1441 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution 1 950 ml - -
Solution 2 50 ml - -

Solution 1 #

950
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCO3 5.0 g 5.208 52.037
Na2HPO4 x H2O 0.1 g 0.104 0.734
MgSO4 x 7 H2O 0.5 g 0.521 2.113
Hutner's salts 20.0 ml - -
Gelrite 8.0 g 9.375 -
Distilled water 930.0 ml - -
1 Adjust pH to 9.0, autoclave at 121°C for 20 min.

Solution 2 #

50
Solution occurs in 1 other media (first defined in Medium 1439).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
N-Acetylglucosamine 2 g 40 180.824
Vitamin solution 10 ml - -
Distilled water 40 ml - -
1 Adjust pH to 9.0, filter-sterilize, and add to solution 1.

Vitamin solution #

1000
Solution occurs in 7 other media (first defined in Medium 1436).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 0.2 mg 2.0e-4 1.5e-4
Biotin 4.0 mg 0.004 0.016
Thiamine-HCl x 2 H2O 10.0 mg 0.01 0.027
Calcium pantothenate 10.0 mg 0.01 0.021
Folic acid 4.0 mg 0.004 0.009
Riboflavin 10.0 mg 0.01 0.027
Nicotinamide 10.0 mg 0.01 0.082
p-Aminobenzoic acid 10.0 mg 0.01 0.073
Pyridoxine hydrochloride 20.0 mg 0.02 0.097
Distilled water 1000.0 ml - -
1 filter-sterilized

Hutner's salts #

1000
Solution occurs in 2 other media (first defined in Medium 603).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA) 		10.000 g 10 52.318
MgSO4 x 7 H2O 29.700 g 29.7 120.499
CaCl2 x 2 H2O 3.335 g 3.335 22.685
(NH4)6Mo7O24 x 4 H2O 9.250 mg 0.009 -
FeSO4 x 7 H2O 99.000 mg 0.099 0.356
"Metals 44" 50.000 ml - -
Distilled water 950.000 ml - -
1 Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4.

"Metals 44" #

1000
Solution occurs in 34 other media (first defined in Medium 590).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-EDTA 250.0 mg 0.25 0.744
ZnSO4 x 7 H2O 1095.0 mg 1.095 3.808
FeSO4 x 7 H2O 500.0 mg 0.5 1.798
MnSO4 x H2O 154.0 mg 0.154 0.911
CuSO4 x 5 H2O 39.2 mg 0.039 0.157
Co(NO3)2 x 6 H2O 24.8 mg 0.025 0.085
Na2B4O7 x 10 H2O 17.7 mg 0.018 -
Distilled water 1000.0 ml - -
1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.

Metadata

Last modified: 10.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.0
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Gelrite 8.911 -
CaCO3 4.95 49.46
N-Acetylglucosamine 1.98 8.952
MgSO4 x 7 H2O 0.588 2.386
Nitrilotriacetic acid 0.198 1.036
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
pH 10 - 12 1 strain
phyla 1 1 strain
nutrition chemoorganotroph 1 strain
oxygen aerobe 1 strain
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