This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Solution 1 | 950 | ml | - | - | |
Solution 2 | 50 | ml | - | - | |
1 After autoclaving add solution 2 to solution 1. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Peptone | 0.25 | g | 0.263 | - | |
Yeast extract | 0.25 | g | 0.263 | - | |
CaCO3 | 5.00 | g | 5.263 | 52.584 | |
Na2HPO4 x H2O | 0.10 | g | 0.105 | 0.742 | |
MgSO4 x 7 H2O | 0.50 | g | 0.526 | 2.135 | |
Hutner's salts | 20.00 | ml | - | - | |
Gelrite | 9.00 | g | 9.474 | - | |
Distilled water | 930.00 | ml | - | - | |
1 Adjust pH to 9.0 with NaOH, autoclave at 121°C for 20 min. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
N-Acetylglucosamine | 2.0 | g | 40 | 180.824 | |
Ampicillin sodium salt | 0.2 | g | 4 | 10.77 | |
Cycloheximid | 0.2 | g | 4 | 14.217 | |
Vitamin solution | 10.0 | ml | - | - | |
Distilled water | 40.0 | ml | - | - | |
1 Adjust pH to 9.0 with NaOH, filter-sterilized |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Vitamin B12 | 0.2 | mg | 2.0e-4 | 1.5e-4 | |
Biotin | 4.0 | mg | 0.004 | 0.016 | |
Thiamine-HCl x 2 H2O | 10.0 | mg | 0.01 | 0.027 | |
Calcium pantothenate | 10.0 | mg | 0.01 | 0.021 | |
Folic acid | 4.0 | mg | 0.004 | 0.009 | |
Riboflavin | 10.0 | mg | 0.01 | 0.027 | |
Nicotinamide | 10.0 | mg | 0.01 | 0.082 | |
p-Aminobenzoic acid | 10.0 | mg | 0.01 | 0.073 | |
Pyridoxine hydrochloride | 20.0 | mg | 0.02 | 0.097 | |
Distilled water | 1000.0 | ml | - | - | |
1 filter-sterilized |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid
(NTA) |
10.000 | g | 10 | 52.318 | |
MgSO4 x 7 H2O | 29.700 | g | 29.7 | 120.499 | |
CaCl2 x 2 H2O | 3.335 | g | 3.335 | 22.685 | |
(NH4)6Mo7O24 x 4 H2O | 9.250 | mg | 0.009 | 0.007 | |
FeSO4 x 7 H2O | 99.000 | mg | 0.099 | 0.356 | |
"Metals 44" | 50.000 | ml | - | - | |
Distilled water | 950.000 | ml | - | - | |
1 Dissolve the nitrilotriacetic acid. | |||||
2 Adjust pH to 7.0 with KOH (~7.3 g). | |||||
3 Dissolve other salts separately and combine. | |||||
4 Adjust pH to 6.8 with NaOH or H2SO4. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na-EDTA | 250.0 | mg | 0.25 | 0.744 | |
ZnSO4 x 7 H2O | 1095.0 | mg | 1.095 | 3.808 | |
FeSO4 x 7 H2O | 500.0 | mg | 0.5 | 1.798 | |
MnSO4 x H2O | 154.0 | mg | 0.154 | 0.911 | |
CuSO4 x 5 H2O | 39.2 | mg | 0.039 | 0.157 | |
Co(NO3)2 x 6 H2O | 24.8 | mg | 0.025 | 0.085 | |
Na2B4O7 x 10 H2O | 17.7 | mg | 0.018 | - | |
Distilled water | 1000.0 | ml | - | - | |
1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions. |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 9.0 |
Equipment needed: | Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Gelrite | 9 | - |
CaCO3 | 5 | 49.955 |
N-Acetylglucosamine | 2 | 9.041 |
MgSO4 x 7 H2O | 0.594 | 2.41 |
Peptone | 0.25 | - |
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