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DSMZ

DSMZ 1434 . ALTEROMONAS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Methylophaga thalassica DSM 9438

This strain uses the standard medium without modifications.

Growth conditions: 20 °C

Main sol. 1434 #

ml
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 2.0 g 2 15.136
K2HPO4 2.0 g 2 11.483
KH2PO4 1.0 g 1 7.348
NaCl 20.0 g 20 342.231
MgSO4 x 7 H2O 0.3 g 0.3 1.217
Vitamin B12 solution 0.001 g/L 1.0 ml - -
Trace elements 1.0 ml - -
Methanol 10.0 ml 7.92 247.176
Agar 15.0 g 15 44.599
Distilled water 1000.0 ml - -
1 pH 7.0
2 Prepare the medium without methanol and vitamin B12 in closed vessels with a medium to headspace ratio of 1:5 to 1:10. Adjust pH to 7.0 and sterilize. Allow to cool to about 45°C before adding 1mL filter-sterilized Vitamin B12 stock solution (0.1mg/100ml) and 10 mL filter-sterilized methanol per litre medium.

Trace elements #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
MnSO4 x H2O 760.0 mg 0.76 4.497
CuSO4 x 5 H2O 250.0 mg 0.25 1.001
FeSO4 x 7 H2O 280.0 mg 0.28 1.007
ZnSO4 x 7 H2O 1.4 g 1.4 4.869
CaCl2 x 2 H2O 150.0 mg 0.15 1.02
CoCl2 x 6 H2O 240.0 mg 0.24 1.848
Na2MoO4 x 2 H2O 240.0 mg 0.24 0.992
Distilled water 1000.0 ml - -

Vitamin B12 solution 0.001 g/L #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 0.1 mg 1.0e-3 7.4e-4
Distilled water 100.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Filter
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Associated strains

All strains for this medium: 1
Methylophaga thalassica DSM 9438

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 20 342.231
Agar 15 44.599
Methanol 7.92 247.176
(NH4)2SO4 2 15.136
K2HPO4 2 11.483
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain
temperature psychrophilic 1 strain