1431
1431 .
ALKALOPHILIC HALOPHILE MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Halorubrum vacuolatum DSM 8800
This strain uses the standard medium without modifications.
Growth conditions: 37 °C
Main sol. 1431 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 10.00 | g | 10 | - | |
| Casamino acids | 7.50 | g | 7.5 | - | |
| Trisodium citrate | 3.00 | g | 3 | 11.625 | |
| KCl | 2.00 | g | 2 | 26.828 | |
| MgSO4 x 7 H2O | 1.00 | g | 1 | 4.057 | |
| MnCl2 x 4 H2O | 0.36 | mg | 3.6e-4 | 0.002 | |
| FeSO4 x 7 H2O | 50.00 | mg | 0.05 | 0.18 | |
| NaCl | 200.00 | g | 200 | 3422.31 | |
| Na2CO3 | 50.00 | g | 50 | 471.752 | |
| Agar | 2.00 | % | 20 | 59.465 | |
| 1 Liquid medium may be prepared by adding all components together. Agar (2%) medium should be prepared without Na2CO3 and sterilised by autoclaving. Add 20-30 ml of a sterile 10% Na2CO3 solution to the autoclaved medium containing agar so that the final pH is 9.0. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Archaea |
| Medium type: | Complex medium |
| Final pH: | 9.0 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Halorubrum vacuolatum DSM 8800
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 200 | 3422.31 |
| Na2CO3 | 50 | 471.752 |
| Agar | 20 | 59.465 |
| Yeast extract | 10 | - |
| Casamino acids | 7.5 | - |
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