1417
1417 .
MMB MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1417 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 0.15 | g | 0.15 | - | |
| Yeast extract | 0.15 | g | 0.15 | - | |
| Ammonium Sulfate | 0.25 | g | 0.25 | 1.892 | |
| Glucose | 1.00 | g | 1 | 5.551 | |
| Modified Hutner`s Basal Salts Solution | 20.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| Agar , for solid medium | 15.00 | g | 15 | 44.599 | |
| 1 Autoclave at 121 °C for 15 min. | |||||
| Vitamine Solution | 10.00 | ml | - | - | |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| D-Calcium pantothenate | 5.0 | mg | 0.005 | - |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Distilled water | 1000.0 | ml | - | - |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 10.00 | g | 9.524 | 49.826 | |
| MgSO4 x 7 H2O | 29.70 | g | 28.286 | 114.761 | |
| CaCl2 x 2 H2O | 3.34 | g | 3.181 | 21.637 | |
| Ammonium Molybdate | 9.25 | mg | 0.009 | 0.045 | |
| FeSO4 x 7 H2O | 99.00 | mg | 0.094 | 0.339 | |
| Metals "44" | 50.00 | ml | - | - | |
| Distilled water | 950.00 | ml | - | - | |
| 1 Note: Dissolve and neutralize the nitrilotriacetic acid with KOH (7.3 g); add the other ingredients and adjust the pH to 6.6 - 6.8 before bringing the volume to 1.0 L with distilled water. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| EDTA | 0.250 | g | 0.25 | 0.855 | |
| ZnSO4 x 7 H2O | 1.100 | g | 1.1 | 3.825 | |
| FeSO4 x 7 H2O | 0.500 | g | 0.5 | 1.798 | |
| MnSO4 x 7 H2O | 0.154 | g | 0.154 | 1.02 | |
| CuSO4 x 5 H2O | 0.040 | g | 0.04 | 0.16 | |
| Co(NO3)2 x 6 H2O | 0.025 | g | 0.025 | 0.086 | |
| Na2B4O7 x 10 H2O | 0.018 | g | 0.018 | - | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Note: Initially add a few drops of H2SO4 to the distilled water to retard precipitation. | |||||
Metadata
| Last modified: | 24.04.23 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Glucose | 1 | 5.551 |
| MgSO4 x 7 H2O | 0.566 | 2.295 |
| Ammonium Sulfate | 0.25 | 1.892 |
| Nitrilotriacetic acid | 0.19 | 0.997 |
| Show more | ||
