1413
1413 .
AMPHIBACILLUS MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1413 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaHCO3 | 15.0 | g | 15 | 178.557 | |
| Na2CO3 | 95.0 | g | 95 | 896.328 | |
| NaCl | 16.0 | g | 16 | 273.785 | |
| K2HPO4 | 1.0 | g | 1 | 5.741 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Prepare the liquid medium. Dispense the medium into serum bottles. After sterilization pH 10.0. Before inoculation add from sterile stock solutions: | |||||
| MgSO4 | 1.0 | mM | 0.12 | 1 | |
| Yeast extract | 0.5 | g/l | 0.5 | - | |
|
Glucose
(20% v/v) |
2.0 | g/l | 2 | 11.101 | |
| Trace elements solution (Pfennig & Lippert,1966) | 1.0 | ml | - | - | |
Solution occurs in 28
other media (first defined in Medium 1369).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| EDTA | 5.00 | g | 5 | 17.109 | |
| FeSO4 x 7 H2O | 2.20 | g | 2.2 | 7.913 | |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| MnCl2 x 4 H2O | 0.03 | g | 0.03 | 0.152 | |
| H3BO3 | 0.03 | g | 0.03 | 0.485 | |
| CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 | |
| CuCl2 x 2 H2O | 0.03 | g | 0.03 | 0.176 | |
| NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 | |
| Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 pH 3.0-4.0 | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Medium type: | Complex medium |
| Final pH: | 10.0 |
| Edit in Medium builder | |