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DSMZ

DSMZ 1412 . METHYLOTHERMUS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1412 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Mineral Salts Solution for strain HTM55 1000 ml - -
1 or
Mineral Salts Solution for strain IT-4 and IT-9 1000 ml - -
2 Prepare the liquid medium. Adjust to pH 6.2 with HCl or NaOH. Dispense the medium into serum bottles under gassing with N2:CO2 (80:20 v/v) for one minute. 20% methane and 5% air is added to the gas phase
3 Adjust pH to 6.2.
4 Dispense the medium into serum bottles under gassing with N2:CO2 (80:20 v/v) for one minute.
5 20% methane and 5% air is added to the gas phase.
6 Autoclave at 121 °C for 20 min.
7 Before inoculation add from sterile stock solutions:
Vitamin Solution 1 ml/l - -
NaHCO3
(5% w/v) 		50 ml/l 2.5 29.759
CuSO4 x 5 H2O
(0.1 M) 		10 ml/l 0.25 1

Mineral Salts Solution for strain HTM55 #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 3.00 g 3 51.335
KH2PO4 14.00 mg 0.014 0.103
CaCl2 x 2 H2O 80.00 mg 0.08 0.544
MgSO4 x 7 H2O 0.34 g 0.34 1.379
MgCl2 x 6 H2O 0.42 g 0.42 2.066
KCl 33.00 g 33 442.656
NH4Cl 0.25 g 0.25 4.674
NaNO3 0.25 g 0.25 2.941
Na2SiO3 x 9 H2O 0.20 mg 2.0e-4 7.0e-4
Fe2(SO4)3 x n H2O 20.00 mg 0.02 0.05
Na2SeO3 x 5 H2O 0.50 mg 5.0e-4 0.002
NiCl2 x 6 H2O 0.50 mg 5.0e-4 0.004
Trace Element Solution 3.00 ml - -
Vitamin Solution 1.00 ml - -
Distilled water 1000.00 ml - -

Mineral Salts Solution for strain IT-4 and IT-9 #

1004
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 30.00 g 29.881 511.302
KH2PO4 140.00 mg 0.139 1.025
CaCl2 x 2 H2O 800.00 mg 0.797 5.42
MgSO4 x 7 H2O 3.40 g 3.386 13.74
MgCl2 x 6 H2O 4.18 g 4.163 20.479
KCl 0.33 g 0.329 4.409
NH4Cl 0.25 g 0.249 4.655
NaNO3 0.25 g 0.249 2.93
Na2SiO3 x 9 H2O 0.20 mg 2.0e-4 7.0e-4
Fe2(SO4)3 x n H2O 20.00 mg 0.02 0.05
Na2SeO3 x 5 H2O 0.50 mg 5.0e-4 0.002
NiCl2 x 6 H2O 0.50 mg 5.0e-4 0.004
Trace Element Solution 3.00 ml - -
Vitamin Solution 1.00 ml - -
Distilled water 1000.00 ml - -

Trace Element Solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MnSO4 x 2 H2O 0.50 g 0.5 2.673
CoSO4 x 7 H2O 0.18 g 0.18 1.161
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO4 0.01 g 0.01 -
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -

Vitamin Solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
D-Calcium pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 25.07.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.2
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
KCl 33 442.656
NaCl 3 51.335
NaHCO3 2.5 29.759
MgCl2 x 6 H2O 0.42 2.066
MgSO4 x 7 H2O 0.34 1.379
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Cultivation metadata from 3 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 3 strains
oxygen aerobe 1 strain
temperature thermophilic 3 strains
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