1409
1409 .
METHYLOCYSTIS MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Methylocystis bryophila DSM 21852
* Modification: PH 5.8 - 6.2; aerobic with 10-20% methane in the headspace
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 3-7 days
aerobic
22 °C
pH 5.80
Main sol. 1409 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KNO3 | 0.20 | g | 0.2 | 1.978 | |
| KH2PO4 | 0.10 | g | 0.1 | 0.735 | |
| MgSO4 x 7 H2O | 0.05 | g | 0.05 | 0.203 | |
| CaCl2 x 2 H2O | 0.01 | g | 0.01 | 0.068 | |
| NaCl | 0.01 | g | 0.01 | 0.171 | |
| Trace Element Solution | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Prepare the liquid medium. Final pH 5.8-6.2. Dispense the medium into serum bottles with a medium to headspace ratio of about 1:4. Add 20% methane to the gas phase. Sterilize by autoclaving. Incubate the cultures with shaking. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| EDTA | 5.00 | g | 5 | 17.109 |
| CuCl2 x 5 H2O | 0.10 | g | 0.1 | 0.445 |
| FeSO4 x 7 H2O | 2.00 | g | 2 | 7.194 |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 |
| NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 |
| CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 |
| Na2MoO4 | 0.03 | g | 0.03 | 0.146 |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | * 5.8 - 6.2 |
| Edit in Medium builder | |
Gas composition
- 20 % methane
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| KNO3 | 0.2 | 1.978 |
| KH2PO4 | 0.1 | 0.735 |
| MgSO4 x 7 H2O | 0.05 | 0.203 |
| NaCl | 0.01 | 0.171 |
| CaCl2 x 2 H2O | 0.01 | 0.068 |
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