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DSMZ

DSMZ 1397 . GS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Ignavibacterium album DSM 19864

This strain uses the standard medium without modifications.

Growth conditions: 3-7 days anaerobic 45 °C

Main sol. 1397 #

ml
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KCl 0.355 g 0.355 4.762
KH2PO4 0.140 g 0.14 1.029
NH4Cl 0.250 g 0.25 4.674
MgCl2 x 6 H2O 4.000 g 4 19.675
MgSO4 x 7 H2O 3.450 g 3.45 13.997
CaCl2 x 2 H2O 0.140 g 0.14 0.952
NaCl 6.000 g 6 102.669
NaHCO3 5.000 g 5 59.519
Fe(NH4)2(SO4)2 x 6 H2O 2.000 mg 0.002 -
Yeast extract
(Difco, Bacto ) 		2.000 g 2 -
Trypticase peptone
(BBL) 		2.000 g 2 -
Glucose 1.800 g 1.8 9.991
Resazurin 1.000 mg 1.0e-3 0.004
Na2S-cysteine solution 10.000 ml - -
Trace element solution 10.000 ml - -
Vitamin solution 10.000 ml - -
Distilled water 1000.000 ml - -
1 pH 7.0
2 Prepare medium except vitamin solution, NaHCO3 und Na2S-cysteine solution under N2/CO2 gas. Add vitamin solution (filter sterilized), NaHCO3 (filter sterilized) and Na2S-cysteine solution(autoclaved) as sterile stock solutions to sterile medium after autoclaving. Adjust pH to 7.0.

Trace element solution #

1000
Solution occurs in 2 other media (first defined in Medium 1375).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).

Vitamin solution #

1000
Solution occurs in 17 other media (first defined in Medium 315).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine-HCl x 2 H2O 5.0 mg 0.005 0.013
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
D-Calcium pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Na2S-cysteine solution #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Cysteine-HCl 5 g 50 317.219
Na2S x 9 H2O 5 g 50 208.175
Distilled water 100 ml - -
1 Prepare under N2 gas.

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1
Ignavibacterium album DSM 19864

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 5 59.519
MgCl2 x 6 H2O 4 19.675
Yeast extract 2 -
Trypticase peptone 2 -
Glucose 1.8 9.991
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain