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DSMZ

DSMZ 1391 . MMJHSY MEDIUM (modified)

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Piezobacter thermophila DSM 19348

This strain uses the standard medium without modifications.

Growth conditions: 2-3 days 50 °C

Main sol. 1391 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.00 g 20 342.231
K2HPO4 0.09 g 0.09 0.517
KH2PO4 0.07 g 0.07 0.514
CaCl2 x 2 H2O 0.80 g 0.8 5.442
NH4Cl 1.00 g 1 18.695
NaNO3 1.00 g 1 11.765
MgSO4 x 7 H2O 4.00 g 4 16.229
MgCl2 x 6 H2O 3.00 g 3 14.756
KCl 0.33 g 0.33 4.427
FeSO4 x 7 H2O 0.01 g 0.01 0.036
Na2S2O3 x 5 H2O 2.00 g 2 8.058
Fe(III) citrate 0.01 g 0.01 0.041
Yeast extract 2.00 g 2 -
Trace mineral solution 5.00 ml - -
Distilled water 1000.00 ml - -
1 Prepare the medium under N2/CO2 (80%N2, 20%CO2) and fill in , the pH of the medium is adjusted with NaOH to 6.8. After autoclaving, the separately autoclaved, concentrated solutions including each of follows are added to the medium. Then a mix gas (80%H2, 20%CO2) is purged for 5 min.
2 Finally, the mix gas (77%H2, 20%CO2, 3%O2) is compressed into gas phase (>80% volume of the tube or bottle) at 2 atm.
Wolin's vitamin solution 1.00 ml - -
NaHCO3 0.10 % 1 11.904

Trace mineral solution #

1000
Solution occurs in 1 other media (first defined in Medium 1121).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MnSO4 x n H2O 0.50 g 0.5 -
CoSO4 x 7 H2O 0.50 g 0.5 3.226
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 1.00 mg 1.0e-3 0.004
SrCl2 x 6 H2O 0.01 g 0.01 0.038
NaBr 0.01 g 0.01 0.097
KI 0.01 g 0.01 0.039
NiCl2 x 6 H2O 0.10 g 0.1 0.772
Na2SeO3 x 5 H2O 0.10 g 0.1 0.38
Fe2(SO4)3 x n H2O 1.00 g 1 2.501
H2WO4 0.10 g 0.1 0.4
Distilled water 1000.00 ml - -

Wolin's vitamin solution #

1000
Solution occurs in 54 other media (first defined in Medium 141).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 0.021
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -
1 Filtrate (pore size 0.22 µm to sterilize).

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Autoclave, Filter
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Gas composition

  • 77 % H2
  • 20 % CO2
  • 3 % O2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1
Piezobacter thermophila DSM 19348

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 20 342.231
MgSO4 x 7 H2O 4 16.229
MgCl2 x 6 H2O 3 14.756
Na2S2O3 x 5 H2O 2 8.058
Yeast extract 2 -
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature thermophilic 1 strain