1386
1386 .
HYPHOMICROBIUM MEDIUM SAF
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1386 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Mineral Salts Solution | 500.000 | ml | - | - | |
| KH2PO4 | 0.200 | g | 0.2 | 1.47 | |
| NH4Cl | 0.250 | g | 0.25 | 4.674 | |
| KCl | 0.500 | g | 0.5 | 6.707 | |
| CaCl2 x 2 H2O | 0.150 | g | 0.15 | 1.02 | |
| NaCl | 1.000 | g | 1 | 17.112 | |
| MgCl2 x 6 H2O | 0.620 | g | 0.62 | 3.05 | |
| Na2SO4 | 2.840 | g | 2.84 | 19.994 | |
| Trace Element Solution | 1.000 | ml | - | - | |
| Distilled water | 500.000 | ml | - | - | |
| 1 Prepare the liquid medium. Adjust to pH 6.0 – 6.5. Dispense the medium into Hungate tubes. Sterilize by autoclaving. Before inoculation add from sterile stock solutions: | |||||
| Vitamin Solution | 1.000 | ml | - | - | |
|
Methanol
(20 mM) |
0.641 | ml | 4.1e-4 | 0.013 | |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HEPES
(20mM) |
2.383 | g | 4.766 | 20 | |
| Distilled water | 500.000 | ml | - | - | |
| 1 pH 6.8 | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
|
HCl
(25%) |
7.700 | g | 7.7 | 211.19 |
| FeSO4 x 7 H2O | 1.500 | g | 1.5 | 5.395 |
| ZnCl2 | 0.070 | g | 0.07 | 0.514 |
| MnCl2 x 4 H2O | 0.100 | g | 0.1 | 0.505 |
| H3BO3 | 0.300 | g | 0.3 | 4.851 |
| CoCl2 x 6 H2O | 0.190 | g | 0.19 | 1.463 |
| CuCl2 x 2 H2O | 2.000 | mg | 0.002 | 0.012 |
| NiCl2 x 6 H2O | 0.024 | g | 0.024 | 0.185 |
| Na2MoO4 x 2 H2O | 0.036 | g | 0.036 | 0.149 |
| Distilled water | 1000.000 | ml | - | - |
Equipment needed: Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| 4-Aminobenzoic Acid | 4.00 | mg | 0.04 | 0.292 |
| B12 | 5.00 | mg | 0.05 | 0.037 |
| D-(+)-biotin | 1.00 | mg | 0.01 | 0.041 |
| Nicotinic acid | 0.01 | g | 0.1 | 0.812 |
| Calcium D-(+)-pantothenate | 5.00 | mg | 0.05 | - |
| Pyridoxamine Dihydrochloride | 0.01 | g | 0.1 | 0.415 |
| Thiamine HCl | 0.01 | g | 0.1 | 0.296 |
| Distilled water | 100.00 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Medium type: | Defined medium |
| Final pH: | 6.0 - 6.5 |
| Equipment needed: | Hungate tubes |
| Edit in Medium builder | |