MediaDive DSMZ Digital Diversity
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder SPARQL endpoint Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 1375 . HALANAEROBACTER JERIDENSE MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Medium for Halanaerobacter jeridensis DSM 23230

This strain uses the standard medium without modifications.

Growth conditions: 1-2 days anaerobic 40 °C

Main sol. 1375 #

ml
Equipment needed: Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.0 g 1 18.695
MgCl2 x 6 H2O 10.0 g 10 49.188
MgSO4 x 7 H2O 5.0 g 5 20.286
KCl 2.0 g 2 26.828
K2HPO4 x 3 H2O 0.5 g 0.5 2.871
NaCl 150.0 g 150 2566.74
Resazurin 1.0 mg 1.0e-3 0.004
Trace element solution 10.0 ml - -
Distilled water 1000.0 ml - -
Glucose
(10% v/v) 		10.0 ml 15.44 85.702
Na2CO3
(10% v/v) 		10.0 ml 25.3 238.706
Na2S x 9 H2O
(2% v/v) 		10.0 ml - -
Yeast extract
(10% v/v) 		10.0 ml - -
L-Cysteine
(10% v/v) 		10.0 ml - -
1 Final pH 8.3
2 Prepare the medium without the Na2S, Na2CO3, NaHCO3, L-cysteine and substrates. Boil the medium and cool under a stream of nitrogen. Dispense the medium into Hungate tubes or serum bottles under gassing with N2. Sterilize by autoclaving. Before inoculation add from sterile stock solutions:

Trace element solution #

1000
Solution occurs in 2 other media (first defined in Medium 1375).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.3
Equipment needed: Hungate tubes
Edit in Medium builder

Associated strains

All strains for this medium: 2
Halanaerobacter jeridensis DSM 23230

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2CO3 25.3 238.706
Glucose 15.44 85.702
MgCl2 x 6 H2O 10 49.188
KCl 2 26.828
NH4Cl 1 18.695
Show more
CSV
JSON

Cultivation metadata from 2 strains BacDive

pH 4 - 6 1 strain
pH 8 - 10 1 strain
phyla 1 2 strains
oxygen anaerobe 1 strain
temperature thermophilic 2 strains
Show more