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DSMZ

DSMZ 1374 . IGNAVIBACTERIA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1374 #

ml
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 1.00 g 1 17.112
MgCl2 x 6 H2O 0.40 g 0.4 1.968
KCl 0.50 g 0.5 6.707
CaCl2 x 2 H2O 0.10 g 0.1 0.68
NH4Cl 0.25 g 0.25 4.674
KH2PO4 0.20 g 0.2 1.47
Na2SO4 4.00 g 4 28.161
Trace element solution 1.00 ml - -
Yeast extract 2.00 g 2 -
NaHCO3 0.10 g 0.1 1.19
Distilled water 990.00 ml - -
1 Autoclave at 121 °C for 15 min.
2 Add filter-sterilized vitamin solution (1ml/10ml)
Vitamin solution 10.00 ml - -

Trace element solution #

1000
Solution occurs in 1 other media (first defined in Medium 1374).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Fe(NH4)2(SO4)2 x 6 H2O 784.0 mg 0.784 -
ZnSO4 x 7 H2O 143.6 mg 0.144 0.499
MnCl2 x 4 H2O 99.0 mg 0.099 0.5
Na2SeO4 94.6 mg 0.095 0.501
H3BO3 6.0 mg 0.006 0.097
Na2WO4 x 2 H2O 33.0 mg 0.033 0.1
CuCl2 x 2 H2O 1.8 mg 0.002 0.011
CoCl2 x 6 H2O 238.0 mg 0.238 1.833
(NH4)2Ni(SO4)2 x 6 H2O 395.0 mg 0.395 1
Na2MoO4 x 2 H2O 24.0 mg 0.024 0.099
HCl
(concentrated) 		10.0 ml - -
Distilled water 990.0 ml - -

Vitamin solution #

1000
Solution occurs in 17 other media (first defined in Medium 315).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine-HCl x 2 H2O 5.0 mg 0.005 0.013
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
D-Calcium pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 21.02.24
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.2 - 7.8
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2SO4 4 28.161
Yeast extract 2 -
NaCl 1 17.112
KCl 0.5 6.707
MgCl2 x 6 H2O 0.4 1.968
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
nutrition organotroph 1 strain
oxygen facultative anaerobe 1 strain
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