1373
1373 .
IRD MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Thermanaerothrix daxensis DSM 23592
This strain uses the standard medium without modifications.
Growth conditions: 7-14 days
60 °C
Main sol. 1373 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.3 | g | 0.3 | 2.205 | |
| K2HPO4 | 0.3 | g | 0.3 | 1.722 | |
| NaCl | 1.0 | g | 1 | 17.112 | |
| NH4Cl | 1.0 | g | 1 | 18.695 | |
| MgCl2 x 6 H2O | 0.5 | g | 0.5 | 2.459 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.68 | |
| KCl | 0.1 | g | 0.1 | 1.341 | |
| Yeast extract | 1.0 | g | 1 | - | |
| Tryptone | 1.0 | g | 1 | 14.069 | |
| Cysteine HCl | 0.5 | g | 0.5 | 4.127 | |
| Modified Wolin's mineral solution II | 1.0 | ml | - | - | |
|
Resazurin
(0.1%) |
0.5 | ml | 5.0e-4 | 0.002 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Autoclave and add the following components after autoclaving from sterile stock solutions: | |||||
|
NaHCO3
(10%) |
10.0 | ml | 1 | 11.904 | |
|
Na2S
(2%) |
10.0 | ml | 0.2 | 2.53 | |
|
Glucose
(1 M) |
10.0 | ml | 1.802 | 10 | |
| 2 pH 7.2 | |||||
| 3 Prepare the medium under a N2/CO2 atmosphere. | |||||
| 4 The final pH of the medium should be 7.2. | |||||
Solution occurs in 24
other media (first defined in Medium 700).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.500 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.000 | g | 3 | 12.172 | |
| MnSO4 x H2O | 0.500 | g | 0.5 | 2.958 | |
| NaCl | 1.000 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.100 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.180 | g | 0.18 | 1.161 | |
| CaCl2 x 2 H2O | 0.100 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.180 | g | 0.18 | 0.626 | |
| CuSO4 x 5 H2O | 0.010 | g | 0.01 | 0.04 | |
| AlK(SO4)2 x 12 H2O | 0.020 | g | 0.02 | 0.042 | |
| H3BO3 | 0.010 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.010 | g | 0.01 | 0.041 | |
| NiCl2 x 6 H2O | 0.025 | g | 0.025 | 0.193 | |
| Na2SeO3 x 5 H2O | 0.300 | mg | 3.0e-4 | 0.001 | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Thermanaerothrix daxensis DSM 23592
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Glucose | 1.802 | 10 |
| NH4Cl | 1 | 18.695 |
| Tryptone | 1 | 14.069 |
| NaHCO3 | 1 | 11.904 |
| Yeast extract | 1 | - |
| Show more | ||
