137
137 .
MINERAL LACTATE MEDIUM (low carbon content)
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 137 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na-lactate | 0.50 | g | 0.5 | 4.462 | |
| K2HPO4 x 3 H2O | 1.13 | g | 1.13 | 6.488 | |
| KH2PO4 | 0.88 | g | 0.88 | 6.467 | |
| NH4Cl | 1.00 | g | 1 | 18.695 | |
| MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| CaCl2 x 2 H2O | 5.00 | mg | 0.005 | 0.034 | |
| Trace element solution (Vishniac & Santer, 1957) | 1.20 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 7.0. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2-EDTA | 50.00 | g | 50 | 148.718 | |
| ZnSO4 x 7 H2O | 22.00 | g | 22 | 76.506 | |
| CaCl2 x 2 H2O | 5.54 | g | 5.54 | 37.683 | |
| MnCl2 x 4 H2O | 5.06 | g | 5.06 | 25.568 | |
| FeSO4 x 7 H2O | 5.00 | g | 5 | 17.985 | |
| (NH4)6Mo7O24 x 4 H2O | 1.10 | g | 1.1 | 0.89 | |
| CuSO4 x 5 H2O | 1.57 | g | 1.57 | 6.288 | |
| CoCl2 x 6 H2O | 1.61 | g | 1.61 | 12.4 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve EDTA in distilled water, adjust pH to 7 using 2 N KOH, then add remaining compounds. Adjust pH of final solution to 6.0 with KOH. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 7.0 |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| K2HPO4 x 3 H2O | 1.13 | 6.488 |
| NH4Cl | 1 | 18.695 |
| NaCl | 1 | 17.112 |
| KH2PO4 | 0.88 | 6.467 |
| Na-lactate | 0.5 | 4.462 |
| Show more | ||
