This strain uses the standard medium without modifications.
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
D-Glucose | 10 | g | ||
|
Glycerol | 10 | g | ||
|
Bacto peptone
(Difco) |
10 | g | ||
|
Yeast extract
(Difco) |
5 | g | ||
|
Ethanol | 10 | ml | ||
|
Distilled water | 1000 | ml | ||
|
Agar , for solid medium | 15 | g | ||
1 Make up the medium without the ethanol, adjust to pH 7.0 and sterilize by autoclaving. Add sterile ethanol to the sterile cooled medium. The medium may be solidified by adding 15.0 g/l agar. |