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DSMZ

DSMZ 1340 . SEHEL FOR HALANAEROBIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Halanaerobium sehlinense DSM 25582

This strain uses the standard medium without modifications.

Growth conditions: anaerobic 40 °C

Main sol. 1340 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.0 g 1 18.695
K2HPO4 0.3 g 0.3 1.722
KH2PO4 0.3 g 0.3 2.205
KCl 0.1 g 0.1 1.341
MgCl2 x 7 H2O 0.5 g 0.5 5.252
CaCl2 x 2 H2O 0.1 g 0.1 0.68
NaCl 200.0 g 200 3422.31
KNO3 2.0 g 2 19.782
Trace Elements Solution 10.0 ml - -
Resazurin 1.0 mg 1.0e-3 0.004
Distilled water 1000.0 ml - -
1 Prepare the liquid medium. Adjust to pH 8.3 with 10 M KOH solution. Boil the medium in a water bath and cool under N2:CO2 (80:20 v/v) to room temperature. Dispense the medium into Hungate tubes or serum bottles under gassing with N2:CO2 (80:20 v/v). Sterilize by autoclaving. Before inoculation add from sterile stock solutions:
NaHCO3
(10% w/v) 		20.0 ml/l 2 23.808
Na2S x 9 H2O
(2% w/v) 		20.0 ml/l 0.4 1.665
MgCl2 x 6 H2O
(10% w/v) 		20.0 ml/l 2 9.838
Glucose
(1M) 		20.0 ml/l 3.603 20
Yeast extract
(10% w/v) 		10.0 ml/l 1 -
Cysteine-HCl
(10% w/v) 		5.0 ml/l 0.5 3.172

Trace Elements Solution #

1000
Equipment needed: Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.03 mg 3.0e-5 1.1e-4
Distilled water 1000.00 ml - -

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.3
Equipment needed: Hungate tubes
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Associated strains

All strains for this medium: 1
Halanaerobium sehlinense DSM 25582

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Glucose 3.603 20
NaHCO3 2 23.808
KNO3 2 19.782
MgCl2 x 6 H2O 2 9.838
NH4Cl 1 18.695
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
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