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DSMZ

DSMZ 1337 . METHYLOPHAGA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Medium for Methylophaga frappieri DSM 25690

This strain uses the standard medium without modifications.

Growth conditions: 28 °C

Main sol. 1337 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 24.0 g 24 410.678
MgCl2 x 6 H2O 3.0 g 3 14.756
MgSO4 x 7 H2O 2.0 g 2 8.114
KCl 0.5 g 0.5 6.707
CaCl2 1.0 g 1 9.011
Distilled water 1000.0 ml - -
Agar , for solid medium 20.0 g 20 59.465
1 Adjust to pH 8.0 and sterilize by autoclaving. The medium may be solidified by adding 20.0 g/l agar.
2 After autoclaving cool down and add the following to 1 litre medium. Methanol and Vitamine B12 are filter sterilized. Solution T and Wolfe's Mineral Solution are separately autoclaved. Final pH 8.0.
Methanol 3.0 ml 2.376 74.153
Solution T 20.0 ml - -
Wolfe's Mineral Solution 10.0 ml - -
Vitamin B12 solution 1.0 ml - -

Solution T #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 7 g 7 51.438
NH4Cl 100 g 100 1869.51
Bis-Tris 100 g 100 477.92
Ferric ammonium citrate 3 g 3 -
Distilled water 1000 ml - -
1 Adjust to pH 8.0 and sterilize by autoclaving.

Wolfe's Mineral Solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 3.00 g 3 24.923
MnSO4 0.50 g 0.5 3.311
NaCl 1.00 g 1 17.112
FeSO4 0.10 g 0.1 0.658
CaCl2 0.10 g 0.1 0.901
CoCl2 0.10 g 0.1 0.77
ZnSO4 0.10 g 0.1 0.62
CuSO4 0.01 g 0.01 0.063
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 0.01 g 0.01 0.049
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals.
2 Adjust to pH 8.0 with KOH and sterilize by autoclaving.

Vitamin B12 solution #

100
Solution occurs in 7 other media (first defined in Medium 28).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 0.01 g - -
Distilled water 100.00 ml - -
1 Filter sterilized

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 8.0
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 2
Methylophaga frappieri DSM 25690

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 20 59.465
MgCl2 x 6 H2O 3 14.756
Methanol 2.376 74.153
NH4Cl 2 37.39
Bis-Tris 2 9.558
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Cultivation metadata from 2 strains BacDive

pH 10 - 12 2 strains
phyla 1 2 strains
oxygen aerobe 2 strains
temperature mesophilic 2 strains