MediaDive DSMZ Digital Diversity
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder SPARQL endpoint Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 1321 . ACIDIHALOBACTER FERROOXIDANS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Acidihalobacter ferrooxydans DSM 14175

This strain uses the standard medium without modifications.

Growth conditions: 2-3 days 35 °C

Main sol. 1321 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 900 ml - -
Solution B 100 ml - -
Solution C 10 ml - -
Solution D 10 ml - -
1 Combine solution A and solution B after cooling and add solutions C and D to complete the medium.
2 Note: Use 10% (v/v) as inoculum.

Solution A #

900
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 15.0 g 16.667 285.193
(NH4)2SO4 0.2 g 0.222 1.682
MgSO4 x 7 H2O 0.4 g 0.444 1.803
K2HPO4 0.1 g 0.111 0.638
Distilled water 900.0 ml - -
1 Adjust the pH to 1.7 with H2SO4 and autoclave at 121°C for 20 min.

Solution B #

100
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 13.9 g 139 499.973
Distilled water 100.0 ml - -
1 Adjust the pH to 1.25 and autoclave solution at 115°C for 10 min or sterilize by filtration.

Solution C #

10
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2S4O6 0.3 g 30 -
Distilled water 10.0 ml - -
1 Sterilize by filtration.

Solution D #

10
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.1 g 10 -
Distilled water 10.0 ml - -
1 Autoclave at 121°C for 20 min.

Metadata

Last modified: 04.05.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave, Filter
Edit in Medium builder

Associated strains

All strains for this medium: 2
Strains with modifications: 1
Acidihalobacter ferrooxydans DSM 14175

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 14.706 251.641
FeSO4 x 7 H2O 13.628 49.017
MgSO4 x 7 H2O 0.392 1.591
K2S4O6 0.294 -
(NH4)2SO4 0.196 1.484
Show more
CSV
JSON

Cultivation metadata from 2 strains BacDive

halophily halotolerant 1 strain
pH 0 - 2 1 strain
pH 2 - 4 1 strain
phyla 1 2 strains
nutrition chemolithoautotroph 1 strain
Show more