1311
1311 .
XTC-2 MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
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Medium for Diplorickettsia massiliensis DSM 23381
This strain uses the standard medium without modifications.
Growth conditions: 28 °C
Equipment needed: Filter
| 1 Cultivation of XTC-2 (from Xenopus laevis) cells | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Leibovitz's L-15 medium | 93 | ml | - | - | |
| Fetal bovine serum | 5 | ml | - | - | |
| Tryptose-phosphate | 2 | ml | - | - | |
| Glutamine | 2 | mM | - | - | |
| 2 Filter-sterilize (0.2 µm) and keep no longer than 4 weeks. Store at room temperature to facilitate detection of contamination. | |||||
| 3 Prepare a 25 cm2 flask and seed cells according to standard protocols. Incubate at 28 - 32°C in hermetic flasks. Cells are easily detached mechanically, no enzyme is necessary. Medium should be changed once a week. When a confluent layer has formed, infection can be carried out. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Leibovitz's L-15 medium | 96 | ml | - | - | |
| Fetal bovine serum | 2 | ml | - | - | |
| Tryptose-phosphate | 2 | ml | - | - | |
| Glutamine | 2 | mM | - | - | |
| 1 Infect cells with 1 ml of Diplorickettsia stock solution (thawed quickly to 37°C). Incubate for 1 h at room temperature. Cultivation temperature: 28°C. | |||||
| 2 Up to 100% of the cells should be infected after several days. The infection level is evaluated after 3 days by Cytospin and Gimenez staining. Cytopathic effects may be visible after 3-5 days. Specific PCRs can be performed to verify the presence of diplorickettsiae. | |||||
Metadata
| Last modified: | 24.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Diplorickettsia massiliensis DSM 23381
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Leibovitz's L-15 medium | - | - |
| Fetal bovine serum | - | - |
| Tryptose-phosphate | - | - |
