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DSMZ

DSMZ 1308 . PY4S

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1308 #

ml
Equipment needed: Gassing station Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trypticase peptone 10.00 g 10 -
Yeast extract 5.00 g 5 -
Glucose 0.25 g 0.25 1.388
Cellobiose 0.25 g 0.25 0.73
Maltose 0.25 g 0.25 0.73
Starch
(soluble) 		0.25 g 0.25 -
L-Cysteine HCl x H2O 0.50 g 0.5 2.847
Sodium resazurin
(0.1% w/v) 		1.00 ml 1.0e-3 -
Solution I 75.00 ml - -
Solution II 75.00 ml - -
Na2CO3
(8% w/V) 		2.50 ml 0.2 1.887
Vitamin B12 10.00 µg 1.0e-5 7.4e-6
Distilled water 846.50 ml - -
1 The cysteine, Na2CO3, and vitamin B12 are added after the medium has been boiled and cooled under CO2. Adjust pH to 7.3 Distribute under N2 and autoclave.

Solution I #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.6 g 6 34.448
Distilled water 100.0 ml - -

Solution II #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCl2 x 2 H2O 0.12 g 1.2 8.162
MgSO4 x 7 H2O 0.12 g 1.2 4.869
KH2PO4 0.60 g 6 44.09
(NH4)2SO4 1.20 g 12 90.813
NaCl 1.20 g 12 205.339
Distilled water 100.00 ml - -

Metadata

Last modified: 27.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.3
Equipment needed: Autoclave, Gassing station
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Trypticase peptone 10 -
Yeast extract 5 -
NaCl 0.9 15.4
(NH4)2SO4 0.9 6.811
L-Cysteine HCl x H2O 0.5 2.847
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
oxygen obligate aerobe 1 strain
temperature mesophilic 1 strain