12 .
SOIL EXTRACT MEDIUM
What is this page?
Equipment needed:
Centrifuge
Final pH:
6.8 - 7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
air-dried garden soil | 400 | g | ||
|
|
Tap water | 1000 | ml | ||
|
|
Agar | 15 | g | ||
| 1 Sterilize 400.0 g of air-dried garden soil (with high content of organic matter) in 1000 ml tap water for one hour at 121°C. Allow it to sediment for a few hours at room temperature. Centrifuge the supernatant. Add 15.0 g agar per 1000 ml to the clear supernatant solution thus obtained. Adjust pH to 6.8 - 7.0 and sterilize. | |||||