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DSMZ

DSMZ 1298 . SULFUROSPIRILLUM ALKALITOLERANS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1298 #

ml
Equipment needed: Autoclave Hungate tubes Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 1.00 g 1 17.112
K2HPO4 1.00 g 1 5.741
NaHCO3 32.00 g 32 380.921
Na2CO3 10.60 g 10.6 100.011
KNO3 2.00 g 2 19.782
MgCl2 x 6 H2O 0.20 g 0.2 0.984
Trace elements solution (Pfennig, 1965) 1.00 ml - -
Yeast extract 0.02 g 0.02 -
Seven vitamins solution 1.00 ml - -
Na-DL-lactate 2.20 g 2.2 19.632
Na2S x 9 H2O 0.10 g 0.1 0.416
Distilled water 1000.00 ml - -
1 Dissolve sodium chloride and hydrogenphospate, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve carbonates, then dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add nitrate, magnesium chloride, trace elements, yeast extract, vitamins, lactate and sulfide from sterile anoxic stock solution prepared under 100% N2 gas. Vitamins should be sterilized by filtration. Adjust pH of complete medium to 9.0, if necessary.

Trace elements solution (Pfennig, 1965) #

1000
Solution occurs in 28 other media (first defined in Medium 1369).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.20 g 2.2 7.913
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.03 g 0.03 0.485
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.03 g 0.03 0.176
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 pH 3.0-4.0

Seven vitamins solution #

1000
Solution occurs in 77 other media (first defined in Medium 503).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
D-(+)-biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 1.459
Thiamine-HCl x 2 H2O 200 mg 0.2 0.536
Distilled water 1000 ml - -

Metadata

Last modified: 03.05.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 32 380.921
Na2CO3 10.6 100.011
Na-DL-lactate 2.2 19.632
KNO3 2 19.782
NaCl 1 17.112
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen anaerobe 1 strain
temperature mesophilic 1 strain