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DSMZ

DSMZ 1297 . NATRONIELLA SULFIDIGENA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1297 #

ml
Equipment needed: Autoclave Water bath Filter Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 25.00 g 24.925 296.704
Na2CO3 140.00 g 139.581 1316.95
NaCl 15.00 g 14.955 255.906
K2HPO4 1.00 g 0.997 5.724
NH4Cl 0.20 g 0.199 3.728
MgCl2 x 6 H2O 0.20 g 0.199 0.981
Yeast extract 0.20 g 0.199 -
Trace elements solution (Pfennig, 1965) 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Glycerol 2.00 g 1.994 21.653
Wolin's vitamin solution (10x) 1.00 ml - -
Sulfur
(powdered) 		2.00 g 1.994 62.177
Na2S x 9 H2O 0.25 g 0.249 1.038
Distilled water 1000.00 ml - -
1 Dissolve hydrogen carbonate, carbonate, sodium chloride, and hydrogen phosphate, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense solution under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add ammonium chloride, magnesium chloride, yeast extract, trace elements, glycerol, vitamins, and sulfide from a sterile anoxic stock solution prepared under 100% N2 gas. The vitamin solution should be sterilized by filtration. Sulfur is sterilized by steaming for 3 hours on each of 3 successive days (see medium 35) and added aseptically to the sterile medium as powder while retaining anoxic conditions. Adjust the pH of the complete medium to 9.8 - 10.0, if necessary.

Trace elements solution (Pfennig, 1965) #

1000
Solution occurs in 28 other media (first defined in Medium 1369).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.20 g 2.2 7.913
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.03 g 0.03 0.485
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.03 g 0.03 0.176
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 pH 3.0-4.0

Selenite-tungstate solution #

1000
Solution occurs in 180 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Wolin's vitamin solution (10x) #

1000
Solution occurs in 307 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 24.07.23
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.8 - 10.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes, Water bath
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2CO3 139.581 1316.95
NaHCO3 24.925 296.704
NaCl 14.955 255.906
Sulfur 1.994 62.177
Glycerol 1.994 21.653
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain