1285 .
GRANULICELLA PALUDICOLA MEDIUM
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* Medium for Acidicapsa acidiphila DSM 29819
Add SL-10 (1 ml/L) (see medium 1426)
Growth conditions: 30 °C
Equipment needed:
Final pH:
4.0 - 5.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Fructose | 0.50 | g | ||
|
|
Yeast extract | 0.05 | g | ||
|
|
Casamino acids | 0.05 | g | ||
|
|
MgSO4 x 7 H2O | 0.04 | g | ||
|
|
CaCl2 x 2 H2O | 0.02 | g | ||
|
|
KH2PO4 | 0.10 | g | ||
|
|
(NH4)2SO4 | 0.10 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
|
|
Agar , for solid medium | 15.00 | g | ||
|
|
Trace element solution SL-10 | 1.00 | ml | ||
| 1 The medium may be solidified with 15 g/l agar. Adjust to pH 4.0 - 5.0 with alginic acid. | |||||
Trace element solution SL-10 * #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
HCl
(25%) |
10.0 | ml | ||
|
|
FeCl2 x 4 H2O | 1.5 | g | ||
|
|
ZnCl2 | 70.0 | mg | ||
|
|
MnCl2 x 4 H2O | 100.0 | mg | ||
|
|
H3BO3 | 6.0 | mg | ||
|
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
CuCl2 x 2 H2O | 2.0 | mg | ||
|
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
|
Distilled water | 990.0 | ml | ||
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||