1279
1279 .
HELICOBACTER MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1279 #
ml
Equipment needed: Hungate tubes
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Brucella Broth
(Becton Dickinson) |
2.80 | g | 35 | - | |
| Demineralized water | 80.00 | ml | - | - | |
| 1 Add aseptically to each tube: | |||||
|
Fetal bovine serum
(heat inactivated) |
2.00 | ml | - | - | |
|
Vitox
(Oxoid) |
0.06 | ml | - | - | |
|
Skirrow supplement
(Oxoid) |
0.02 | ml | - | - | |
|
Amphotericin
(10 mg/ml) |
0.02 | ml | - | - | |
| 2 Adjust with HCl to pH 5.0, make anoxic under a stream of N2/CO2, distribute in Hungate tubes to 8 ml and autoclave. | |||||
| 3 Add 1 ml of sterile air to each tube. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 5.0 |
| Equipment needed: | Autoclave, Hungate tubes |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Brucella Broth | 35 | - |
| Skirrow supplement | - | - |
| Vitox | - | - |
| Fetal bovine serum | - | - |
| Demineralized water | - | - |
| Show more | ||
Cultivation metadata from 10 strains
| phyla | 1 | 10 strains |
| oxygen | microaerophile | 3 strains |
| oxygen | anaerobe | 1 strain |
| temperature | mesophilic | 10 strains |