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DSMZ

DSMZ 1266a . MEDIUM GY VL55

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1266a #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution 1 500 ml - -
Solution 2 500 ml - -
1 After autoclaving, cool to 50-60°C and combine the solution by pouring solution 2 to solution 1.

Solution 1 #

492
Compound Amount Unit Conc. [g/L] Conc. [mM]
MES
(2-Morpholinoethanesulfonic acid) 		1.95 g 3.963 20.3
20 mM MgSO4 x 7 H2O 10.00 ml 0.049 0.2
30 mM CaCl2 x 2 H2O 10.00 ml 0.044 0.3
20mM (NH4)2HPO4 10.00 ml 0.026 0.2
Selenite-tungstate solution 1.00 ml - -
Trace element solution SL-10 1.00 ml - -
Distilled water 460.00 ml - -
1 Adjust to pH 5.5 with NaOH/KOH solution (see below).

Solution 2 #

500
Compound Amount Unit Conc. [g/L] Conc. [mM]
Glucose 1.0 g 2 11.101
Yeast extract 0.5 g 1 -
Distilled water 500.0 ml - -
Agar , for solid medium 20.0 g 40 118.931

20 mM MgSO4 x 7 H2O #

100
Solution occurs in 1 other media (first defined in Medium 1266).
Compound Amount Unit Conc. [g/L] Conc. [mM]
H2O 100.00 ml - -
MgSO4 x 7 H2O 0.49 g 4.9 19.88

30 mM CaCl2 x 2 H2O #

100
Solution occurs in 1 other media (first defined in Medium 1266).
Compound Amount Unit Conc. [g/L] Conc. [mM]
H2O 100.00 ml - -
CaCl2 x 2 H2O 0.44 g 4.4 29.929

20mM (NH4)2HPO4 #

100
Solution occurs in 1 other media (first defined in Medium 1266).
Compound Amount Unit Conc. [g/L] Conc. [mM]
H2O 100.00 ml - -
(NH4)2HPO4 0.26 g 2.6 19.688

NaOH/KOH #

100
Solution occurs in 1 other media (first defined in Medium 1266).
Compound Amount Unit Conc. [g/L] Conc. [mM]
H2O 100.00 ml - -
NaOH 0.80 g 8 200.015
KOH 0.56 g 5.6 99.811

Selenite-tungstate solution #

1000
Solution occurs in 178 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Trace element solution SL-10 #

1000
Solution occurs in 265 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 5.5
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 20 59.466
MES 1.982 10.15
Glucose 1 5.551
Yeast extract 0.5 -
MgSO4 x 7 H2O 0.05 0.202
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain