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DSMZ

DSMZ 1266 . MEDIUM VL55

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1266 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Liquid media 1000 ml - -
Solid media 1000 ml - -

Liquid media #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
MES
(2-Morpholinoethanesulfonic acid) 		1.95 g 1.95 9.988
MgSO4 x 7 H2O
(20 mM) 		10.00 ml 0.049 0.2
CaCl2 x 2 H2O
(30 mM) 		10.00 ml 0.044 0.3
(NH4)2HPO4
(20 mM) 		10.00 ml 0.026 0.2
Selenite-tungstate solution 1.00 ml - -
Trace element solution SL-10 1.00 ml - -
Distilled water 960.00 ml - -
1 After autoclaving add:
Vitamin solution 3.00 ml - -
Glucose
(filter-sterilized, 0.2M) 		10.00 ml 0.36 2
Medium 1266 1.00 ml - -
2 Adjust to pH 5.5 with NaOH/KOH solution (see below).

Solid media #

1000
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
MES
(2-Morpholinoethanesulfonic acid) 		3.9 g 3.9 19.975
MgSO4 x 7 H2O
(20 mM) 		20.0 ml 0.099 0.4
CaCl2 x 2 H2O
(30 mM) 		20.0 ml 0.088 0.6
(NH4)2HPO4
(20 mM) 		20.0 ml 0.053 0.4
Selenite-tungstate solution 2.0 ml - -
Trace element solution SL-10 2.0 ml - -
Distilled water 920.0 ml - -
1 After autoclaving, keep at about 60°C, then add to each 250 ml lot:
Vitamin solution 1.5 ml - -
Glucose
(filter-sterilized, 0.2M) 		10.0 ml 0.36 2
Agar
(autoclaved, kept about 60°C, 3%, washed) 		250.0 ml 7.5 22.3
2 Adjust to pH 5.5 with NaOH/KOH solution (see below).
3 Autoclave in 250ml lots, with a magnetic stirring bar in each bottle.

20 mM MgSO4 x 7 H2O #

100
Solution occurs in 1 other media (first defined in Medium 1266).
Compound Amount Unit Conc. [g/L] Conc. [mM]
H2O 100.00 ml - -
MgSO4 x 7 H2O 0.49 g 4.9 19.88

30 mM CaCl2 x 2 H2O #

100
Solution occurs in 1 other media (first defined in Medium 1266).
Compound Amount Unit Conc. [g/L] Conc. [mM]
H2O 100.00 ml - -
CaCl2 x 2 H2O 0.44 g 4.4 29.929

20mM (NH4)2HPO4 #

100
Solution occurs in 1 other media (first defined in Medium 1266).
Compound Amount Unit Conc. [g/L] Conc. [mM]
H2O 100.00 ml - -
(NH4)2HPO4 0.26 g 2.6 19.688

NaOH/KOH #

100
Solution occurs in 1 other media (first defined in Medium 1266).
Compound Amount Unit Conc. [g/L] Conc. [mM]
H2O 100.00 ml - -
NaOH 0.80 g 8 200.015
KOH 0.56 g 5.6 99.811

Selenite-tungstate solution #

1000
Solution occurs in 178 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Trace element solution SL-10 #

1000
Solution occurs in 265 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Vitamin solution #

1000
Solution occurs in 1 other media (first defined in Medium 1266).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 17 mg 0.017 0.013
4-aminobenzoate 13 mg 0.013 0.095
Biotin 3 mg 0.003 0.012
Nicotinic acid 33 mg 0.033 0.268
Hemicalcium D-(+)-pantothenate 17 mg 0.017 -
Pyridoxamine hydrochloride 50 mg 0.05 0.244
Thiamine-HCl x 2 H2O 33 mg 0.033 0.088
DL-6,8-thioctic acid 10 mg 0.01 0.048
Riboflavin 10 mg 0.01 0.027
Folic acid 4 mg 0.004 0.009
Distilled water 1000 ml - -
1 Filter sterilize the solution.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 5.5
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 8

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MES 1.94 9.938
FeCl2 x 4 H2O 0.001 0.008
NaOH 5.0e-4 0.012
CoCl2 x 6 H2O 1.9e-4 0.001
Pyridoxamine hydrochloride 1.5e-4 -
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Cultivation metadata from 8 strains BacDive

pH 6 - 8 1 strain
phyla 2 8 strains
nutrition heterotroph 1 strain
oxygen aerobe 1 strain
temperature mesophilic 8 strains