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DSMZ

DSMZ 1242 . ALGORIPHAGUS ALKALIPHILUS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1242 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 1.00 g 1 -
Proteose peptone no. 3 1.00 g 1 -
Casamino acids 1.00 g 1 -
Glucose 1.00 g 1 5.551
Pyruvic acid 0.05 g 0.05 0.568
MgSO4 x 7 H2O 0.10 g 0.1 0.406
K2HPO4 0.60 g 0.6 3.445
Mineralsolution 1 50.00 ml - -
Traceelements Solution 5.00 ml - -
Tris buffer
(1 M pH 8.0) 		100.00 ml 12.114 100
Agar 15.00 g 15 44.599
Distilled water 845.00 ml - -
1 Adjust pH 7.8-8.2
2 Autoclave at 121°C for 15 minutes.

Mineralsolution 1 #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1000 mg 1 5.232
CaSO4 x 2 H2O 600 mg 0.6 3.485
MgSO4 x 7 H2O 1000 mg 1 4.057
NaCl 800 mg 0.8 13.689
KNO3 1030 mg 1.03 10.188
NaNO3 6890 mg 6.89 81.064
Na2HPO4 1110 mg 1.11 7.819
Distilled water 1000 ml - -

Traceelements Solution #

1000
Solution occurs in 1 other media (first defined in Medium 1033).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MnSO4 x H2O 0.22 g 0.22 1.302
ZnSO4 x 7 H2O 0.05 g 0.05 0.174
H3BO3 0.05 g 0.05 0.809
CuSO4 x 5 H2O 2.50 mg 0.003 0.01
Na2MoO4 x 2 H2O 2.50 mg 0.003 0.01
CoCl2 x 6 H2O 4.60 mg 0.005 0.035
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.8 - 8.2
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Tris buffer 12.114 100
Glucose 1 5.551
Proteose peptone no. 3 1 -
Casamino acids 1 -
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Cultivation metadata from 2 strains BacDive

phyla 2 2 strains
oxygen obligate aerobe 1 strain