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ACIDITHIOBACILLUS FERRIVORANS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
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Medium for Acidithiobacillus ferrivorans DSM 22755
This strain uses the standard medium without modifications.
Growth conditions: 3-7 days
28 °C
Main sol. 1234 #
ml
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2SO4 | 1.40 | g | 1.4 | 9.856 | |
| (NH4)2SO4 | 3.00 | g | 3 | 22.703 | |
| KCl | 0.10 | g | 0.1 | 1.341 | |
| MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 | |
| K2HPO4 | 0.05 | g | 0.05 | 0.287 | |
|
Ca(NO3)2 x 4 H2O
(0.1% w/v) |
20.00 | ml | 0.02 | 0.085 | |
|
(NH4)2Ni(SO4)2 x 6 H2O
(0.1% w/v) |
20.00 | ml | 0.02 | 0.051 | |
| Modified Wolin's mineral solution | 10.00 | ml | - | - | |
| K2S4O6 | 0.80 | g | 0.8 | - | |
| FeSO4 x 7 H2O | 0.03 | g | 0.03 | 0.108 | |
| Distilled water | 950.00 | ml | - | - | |
| 1 Dissolve ingredients (except tetrathionate and ferrous sulfate), adjust pH to 2.5 with H2SO4, and autoclave. After cooling, add potassium tetrathionate from a 100 mM (33.4 g/l) stock solution and ferrous sulfate heptahydrate from a 10 mM (2.78 g/l) stock solution. The ferrous sulfate solution must be prepared in water adjusted to pH 2.0 using H₂SO₄. Both solutions should be freshly prepared and sterilized by filtration. Add appropriate volumes of the stock solutions to achieve final concentrations of 0.8 g/l K₂S₄O₆ and 0.03 g/l FeSO₄ x 7 H₂O in the medium. | |||||
Solution occurs in 168
other media (first defined in Medium 141).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| MnSO4 x H2O | 0.50 | g | 0.5 | 2.958 | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.18 | g | 0.18 | 1.161 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.18 | g | 0.18 | 0.626 | |
| CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
| AlK(SO4)2 x 12 H2O | 0.02 | g | 0.02 | 0.042 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 | |
| Na2SeO3 x 5 H2O | 0.30 | mg | 3.0e-4 | 0.001 | |
| Na2WO4 x 2 H2O | 0.40 | mg | 4.0e-4 | 0.001 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. | |||||
Metadata
| Last modified: | 11.05.26 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 3 |
| Strains with modifications: | 2 |
Acidithiobacillus ferrivorans DSM 22755
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| (NH4)2SO4 | 3 | 22.703 |
| Na2SO4 | 1.4 | 9.856 |
| K2S4O6 | 0.8 | - |
| KCl | 0.1 | 1.341 |
| K2HPO4 | 0.05 | 0.287 |
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