Name: ACIDITHIOBACILLUS FERRIVORANS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 1234 #

Equipment needed: Filter Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Na₂SO₄	1.40	g	1.4	9.856
(NH₄)₂SO₄	3.00	g	3	22.703
KCl	0.10	g	0.1	1.341
MgSO₄ x 7 H₂O	0.50	g	0.5	2.029
K₂HPO₄	0.05	g	0.05	0.287
Ca(NO₃)₂ x 4 H₂O (0.1% w/v)	20.00	ml	0.02	0.085
(NH₄)₂Ni(SO₄)₂ x 6 H₂O (0.1% w/v)	20.00	ml	0.02	0.051
Modified Wolin's mineral solution	10.00	ml	-	-
K₂S₄O₆	0.80	g	0.8	-
FeSO₄ x 7 H₂O	0.03	g	0.03	0.108
Distilled water	950.00	ml	-	-
1 Dissolve ingredients (except tetrathionate and ferrous sulfate), adjust pH to 2.5 with H₂SO₄ and autoclave. After cooling, add tetrathionate from a 100 mM stock solution and FeSO₄ from a 10 mM stock solution (prepared in water adjusted to pH 2.0 with H₂SO₄). Both solutions should be freshly prepared and sterilized by filtration.

Modified Wolin's mineral solution #

1000

Solution occurs in 166 other media (first defined in Medium 141).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Nitrilotriacetic acid	1.50	g	1.5	7.848
MgSO₄ x 7 H₂O	3.00	g	3	12.172
MnSO₄ x H₂O	0.50	g	0.5	2.958
NaCl	1.00	g	1	17.112
FeSO₄ x 7 H₂O	0.10	g	0.1	0.36
CoSO₄ x 7 H₂O	0.18	g	0.18	1.161
CaCl₂ x 2 H₂O	0.10	g	0.1	0.68
ZnSO₄ x 7 H₂O	0.18	g	0.18	0.626
CuSO₄ x 5 H₂O	0.01	g	0.01	0.04
AlK(SO₄)₂ x 12 H₂O	0.02	g	0.02	0.042
H₃BO₃	0.01	g	0.01	0.162
Na₂MoO₄ x 2 H₂O	0.01	g	0.01	0.041
NiCl₂ x 6 H₂O	0.03	g	0.03	0.231
Na₂SeO₃ x 5 H₂O	0.30	mg	3.0e-4	0.001
Na₂WO₄ x 2 H₂O	0.40	mg	4.0e-4	0.001
Distilled water	1000.00	ml	-	-
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

All strains for this medium:	3
Strains with modifications:	2

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