1220 .
ACIDOSOMA MEDIUM
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Selected strain: Acidisoma sibiricum DSM 21000
This strain uses the standard medium without modifications.
Growth conditions: 20 °C
Equipment needed:
Final pH:
5.0 - 5.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 0.10 | g | ||
|
|
(NH4)2SO4 | 0.25 | g | ||
|
|
CaCl2 x 2 H2O | 0.05 | g | ||
|
|
MgSO4 x 7 H2O | 0.02 | g | ||
|
|
Trace elements | 1.00 | ml | ||
|
|
Yeast extract | 0.10 | g | ||
|
|
Na gluconate | 0.50 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
|
|
Agar , for solid medium | 15.00 | g | ||
| 1 Adjust to pH 5.0 - 5.5. Agar may be added at 15 g/l for solid media. | |||||
Trace elements #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
EDTA | 5.00 | g |
|
|
CuCl2 x 5 H2O | 0.10 | g |
|
|
FeSO4 x 7 H2O | 2.00 | g |
|
|
ZnSO4 x 7 H2O | 0.10 | g |
|
|
NiCl2 x 6 H2O | 0.02 | g |
|
|
CoCl2 x 6 H2O | 0.20 | g |
|
|
Na2MoO4 | 0.03 | g |
|
|
Distilled water | 1000.00 | ml |