1212
1212 .
MARINOSPIRILLUM CELERE
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1212 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.5 | g | 0.5 | 9.348 | |
| KH2PO4 | 0.5 | g | 0.5 | 3.674 | |
| NaCl | 25.0 | g | 25 | 427.789 | |
| Na2SO4 | 0.5 | g | 0.5 | 3.52 | |
| MgSO4 x 7 H2O | 0.2 | g | 0.2 | 0.811 | |
| KCl | 0.5 | g | 0.5 | 6.707 | |
| Yeast extract | 1.0 | g | 1 | - | |
| Peptone | 1.0 | g | 1 | - | |
| Sodium DL-lactate | 1.0 | g | 1 | 8.924 | |
| Vitamin B12 | 15.0 | µg | 1.5e-5 | 1.1e-5 | |
| Na2CO3 | 5.0 | g | 5 | 47.175 | |
| NaHCO3 | 5.0 | g | 5 | 59.519 | |
| Trace element solution SL-10 B | 1.0 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| Agar , for solid medium | 15.0 | g | 15 | 44.599 | |
| 1 Autoclave the Na2CO3, NaHCO3 and the lactate separately from the medium and combine after cooling. Vitamin B12 is added from a sterile stock solution. The final pH of the medium should be 9.5. Add 15.0 g/l agar for solid media. | |||||
Solution occurs in 1
other media (first defined in Medium 1200).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Distilled water | 1000.0 | ml | - | - |
|
HCl
(25%) |
7.7 | ml | 1.925 | 52.798 |
| FeSO4 x 7 H2O | 1.5 | g | 1.488 | 5.353 |
| ZnCl2 | 70.0 | mg | 0.069 | 0.509 |
| MnCl2 x 4 H2O | 100.0 | mg | 0.099 | 0.501 |
| H3BO3 | 300.0 | mg | 0.298 | 4.813 |
| CoCl2 x 6 H2O | 190.0 | mg | 0.188 | 1.452 |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.184 |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.148 |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 9.5 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 25 | 427.789 |
| Agar | 15 | 44.599 |
| NaHCO3 | 5 | 59.519 |
| Na2CO3 | 5 | 47.175 |
| Sodium DL-lactate | 1 | 8.924 |
| Show more | ||
